Phenotypic and molecular characterization of F5 and backcross generations for salt tolerance in wheat (Triticum aestivum L. em. Thell)
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Date
2019
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CCSHAU
Abstract
Soil salinity is one of the major abiotic stress having multifarious adverse effects on all growth
stages of wheat. In Haryana, WH 1105 is widely cultivated wheat variety with many agronomically
superior characteristics but is affected by soil salinity. In the present investigationNax1 and Nax2 genes
were introgressed from Kharchia 65 into WH 1105 for salinity tolerance through marker assisted
backcross breeding. F5, BC1F4, BC2F3 and BC3F1 generations of WH 1105 x Kharchia 65, were
evaluated under initial salt stress condition for various morphological traits. On the basis of genotypic
and phenotypic variations 57 high yielding plants of WH 1105 x Kharchia 65, were selected. Out of
total 210 screened SSRs 30 markers were found polymorphic for WH 1105 and Kharchia 65. These
polymorphic SSRs were utilized to produce molecular diversity among selected plants. Cluster tree
analysis of parents and all the four generations of the cross, portrayed that all the selected plants were
inclined toward recurrent parent WH1105 indicating higher similarity with the recurrent parent. Seven
best plants were selected from the cross as high grain yielding and salt tolerant plants. These plants
could be further backcrossed with the recurrent parent to develop salt tolerant wheat lines.
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