Pro-inflammatory cytokine and apoptotic gene mRNA levels against lentogenic and velogenic Newcastle disease virus pathotypes in in-vivo and in-vitro biological systems
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Date
2019-04
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Abstract
Knowledge on the influence of pro-inflammatory cytokine and apoptotic gene mRNA levels in the pathogenesis of Indian
field isolates of Newcastle disease virus (NDV) is little. In this study, cytokine mRNA levels were elucidated in spleen of
chickens (in-vivo) and chicken embryo fibroblast cells (in-vitro) infected with lentogenic D58 strain and viscerotropic
velogenic D165 isolate until five days post infection (dpi). In spleen of chickens infected with D165, maximum upregulation
of pro-inflammatory cytokines (IL-1, IL-6, TNF-), chemokine (IL-8) and apoptotic gene (Caspase-8) at 3dpi correlated
with the onset of severe clinical signs and necrotic histopathological lesions in spleen, proventriculus, intestine and caecal
tonsil of chickens. Similarly, in CEF cells infected with D165, upregulation of pro-inflammatory cytokine and apoptotic
gene mRNA levels correlated with the appearance of CPE. In spleen of chickens and CEF cells infected with D58, there was
comparatively minimal upregulation of pro-inflammatory cytokine and apoptotic gene mRNA levels which did not cause
histopathological changes in tissues and CPE formation in CEF cells. In both in-vivo and in-vitro systems, upregulation of
anti-inflammatory cytokine IL-10 showed inhibitory effects on the mRNA levels of pro-inflammatory cytokines. Thus, this
study reports variation in the cytokine mRNA levels elucidated in response to two different pathotypes isolated from India
and associates the same with the clinical signs and pathological lesions produced during the course of ND.
Description
TNV_IJAR_2019_53(4)515-522
Keywords
Veterinary Science