CLOACAL CARRIAGE AND ANTIMICROBIAL RESISTANCE PROFILING OF EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING ESCHERICHIA COLI AND SALMONELLA ENTERITIDIS IN BROILER CHICKEN

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dc.contributor.advisorC. Latha
dc.contributor.authorAFSAL S.
dc.date.accessioned2023-02-13T10:59:04Z
dc.date.available2023-02-13T10:59:04Z
dc.date.issued2021-12-30
dc.descriptionSubmitted in partial fulfilment of the requirement for the degree of Master of Veterinary Science in Veterinary Public Healthen_US
dc.description.abstractIn the current scenario, foodborne diseases act as a major challenge to the public health, which accounts for high morbidity and mortality in man. The present study was undertaken to determine cloacal carriage and antimicrobial resistance profiling of extended spectrum beta-lactamase producing E. coli and S. Enteritidis in broiler chicken from Kollam and Kottayam districts. The study was conducted over a period of one year from October 2019 to November 2020. Conventional culture techniques followed by PCR confirmation were used to find the occurrence of E. coli and S. Enteritidis. Antibiotic sensitivity pattern of the positive isolates were studied using disc diffusion assay and further molecular confirmation carried out by targeting ß-lactamase resistance genes. The conventional culture techniques revealed an overall occurrence of E. coli and Salmonella spp. in 77.8 and 9.3 per cent samples of broiler chicken respectively, from both districts. Statistical analysis showed no significant difference (p >0.05) between the occurrence of E. coli and Salmonella spp. in cloacal samples of broiler chicken by culture techniques from Kollam and Kottayam districts. The samples which were subjected to conventional culture techniques were further analysed for PCR confirmation. An overall occurrence of 61.8, 7.8 and 3.3 per cent were confirmed for E. coli (uidA), Salmonella spp. (invA) and S. Enteritidis (sen) respectively, by PCR from both districts. In this study, occurrence of PCR confirmed E. coli and S. Enteritidis isolate in broiler chicken from Kollam and Kottayam differ significantly (p0.05) noticed during the analysis of PCR confirmed Salmonella spp. isolates between Kollam and Kottayam districts. All the PCR confirmed E. coli and S. Enteritidis isolates from both Kollam and Kottayam districts were subjected to antibiotic resistance profiling against ß-lactam group of antibiotics by disc diffusion assay. The study revealed that 19.02 per cent E. coli isolates were completely resistant to ampicillin followed by ceftriaxone, cephalexin, cefotaxime, amoxicillin-clavulanic acid, cefixime and cefoxitin. Whereas, S. Enteritidis isolates showed 15.38 per cent resistance to ampicillin followed by 7.69 per cent resistance to ceftriaxone. The MAR index of E. coli isolates was found to be same (0.47) for samples from Kollam and Kottayam districts, whereas for S. Enteritidis isolates 0.13 for samples from Kollam and zero for samples from kottayam. Study on antibiotic resistance genes in E. coli by PCR revealed that, 42.72 and 5.83 per cent out of the 103 ß-lactam antibiotic resistant E. coli isolates recorded genotypic resistance to blaTEM and blaCTX-M genes respectively. None of the isolates were positive for the presence of blaMOX and blaKPC resistance genes. Similiarly, out of the eight ß-lactam antibiotic resistant S. Enteritidis isolates, the study revealed that 25 per cent showed presence of blaTEM gene, none of the isolates showed genotypic resistance to blaCTX-M, blaMOX and blaKPC genes. The results of the present study demonstrate that, poultry act as a potential source of E. coli and S. Enteritidis. The study also highlighted an occurrence of antibiotic resistance against ß-lactam antibiotics. The indiscriminate use of antibiotics in animal, humans and other allied sectors are the major factor contributing for antimicrobial resistance. A coordinated surveillance systems is necessary to report the occurrence of antimicrobial resistance among these zoonotic pathogens to elucidate prevention and control strategies. A multifaceted One Health approach can combat foodborne diseases as well as antimicrobial resistance. This include collaborative approach by various disciplines including human medicine, veterinary medicine, epidemiology, environmental specialist, public health institutes and epidemiological surveillance agencies. A coordinated approach of these disciplines are significant to prevent or control foodborne diseases as well as antimicrobial resistance.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810193741
dc.keywordsCLOACAL CARRIAGE, ANTIMICROBIAL RESISTANCE PROFILING, EXTENDED SPECTRUM BETA-LACTAMASE, ESCHERICHIA COLI, SALMONELLA ENTERITIDIS,BROILER CHICKEN, Isolation and identification of E. coli and Salmonella spp. by conventional techiques, Isolation and identification of Salmonella sp, Occurrence of E. coli in Poultry, Molecular confirmation of E. coli, Salmonella spp. and S. Enteritidis isolates by PCR, Confirmation of E. coli isolates by PCR, Detection of ß-lactamase resistance genes in Salmonella spp.,en_US
dc.language.isoEnglishen_US
dc.pages154 p.en_US
dc.publisherCOLLEGE OF VETERINARY AND ANIMAL SCIENCES MANNUTHY, THRISSURen_US
dc.research.problemOccurrence of E. coli and S. Enteritidis in cloacal samples of broiler chicken by culture technique , Molecular confirmation of the isolates by PCR , Susceptibility testing against ß-lactam group of antibiotics by disc diffusion assay , Detection of ß-lactamase resistance genes by PCRen_US
dc.subVeterinary Public Healthen_US
dc.themeBETA-LACTAMASE PRODUCING ESCHERICHIA COLI AND SALMONELLA ENTERITIDIS IN BROILER CHICKENen_US
dc.these.typeM.V.Sc.en_US
dc.titleCLOACAL CARRIAGE AND ANTIMICROBIAL RESISTANCE PROFILING OF EXTENDED SPECTRUM BETA-LACTAMASE PRODUCING ESCHERICHIA COLI AND SALMONELLA ENTERITIDIS IN BROILER CHICKENen_US
dc.typeThesisen_US
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