Comparative impact of insecticides on bacterial and acetylcholinesterase activity of Eisenia fetida (Savigny) and Pheretima posthuma (Kinberg)
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Date
2017
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CCSHAU
Abstract
The present study was carried out to analyze the toxicity of chlorpyrifos and carbofuran on E. fetida and P.
posthuma. Paper contact toxicity test was used to determine LC50. LC50 of chlorpyrifos against adult E. fetida and
P. posthuma was 0.25% and 0.11%, respectively. While LC50 of carbofuran against adult E. fetida and P.
posthuma was 5.14% and 4.11%, respectively that confirms the greater sensitivity of P. posthuma towards
insecticide induced toxicity in comparison to E. fetida. Lower LC50 value of chlorpyrifos confirms its higher
toxicity as compared with carbofuran. Total 52 bacterial isolates were isolated from the gut of worms, out of
which six resistant gut bacterial species having agricultural importance were identified as Sphingobacterium sp.,
Citrobacter freundii, Citrobacter murliniae, Bacillus australimaris and Burkholderia paludis. Inhibition in
AChE activity due to chlorpyrifos and carbofuran exposure was observed at 24h. While E. fetida exposed to
carbofuran tried to maintain AChE level, which may be seen as strategy to cope up with toxicity after prolonged
exposure.
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