HAPLOTYPE ANALYSIS OF Phosphorus uptake 1 (Pup1) AND ITS RELATIONSHIP WITH PHOSPHORUS DEFICIENCY TOLERANCE IN RICE
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Date
2016
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DIVISION OF GENETICS ICAR-INDIAN AGRICULTURAL RESEARCH INSTITUTE NEW DELHI
Abstract
Imparting low P response to rice varieties has become inevitable to
contain the use of P fertilizers in order get rid of it scarcity, increasing soil P
deficiency and increasing P fertiliser prices and to prevent P related pollution
in high input areas. A rice QTL, Pup1 has been identified to impart low P
tolerance. Pup1 is a complex loci and its structure in Indian rice germplasm is
largely unknown. A study was conducted to analyse the haplotype features
of Pup1 in an 88-line germplasm set containing several indigenous and few
exotic rice lines. The genotypes were screened at seedling stage under
hydroponic screen under two P regimes, -P (0 ppm) and +P (10 ppm). The
genotypes showed significant phenotypic variation in P response under two P
regimes. The root traits had increased values than under -P regime, except for
the root number, whereas shoot traits showed better performance under +P
regimes. Among the root parameters, root length was the major trait for low P
response. The genotypes could be grouped into three groups -P responders,
+P responders and insensitves, based on phenotype performance.
Haplotype analyses revealed that 39.4% of the Kasalath Pup1 locus was
conserved in the germplasm. The conserved region itself is highly fragmented.
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Proportion of ‘null’ alleles were more on an average, spread among the
irrigated varieties. Presence of Kasalath locus in high proportion, however
does not guaranteed good low P response. Few of the good -P responders were
having only little fraction of Kasalath alleles. Several Basmati landraces and
cultivars conserved large proportion of Kasalath allele, the evolutionary
reason can be further investigated. The fragmentation of Pup1 haplotypes is
further revealed by poor degree of marker trait association. The region
encompassed by K46K1, K46-2 and K46-3 which carry OsPSTOL1 was key
region that conformed low P response. The study emphasised that OsPSTOL1
gene be the driving factor for low P tolerance qualifying it for use rice
improvement program and several indigenous sources can be used as the
donor for this gene
Description
t-9425
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