Studies on Variability and Management of Macrophomina phaseolina (Tassi.) Goid. Causing Dry Root Rot of Clusterbean [Cyamopsis tetragonoloba (L.) Taub.]
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Date
2016
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Abstract
Experiments were conducted to study the variability of Macrophomina
phaseolina isolates collected from clusterbean growing regions of Rajasthan and
adjoining places using ten molecular markers and ten clusterbean genotypes.
Ten Trichoderma isolates and six Pseudomonas fluorescens isolates were
studied using molecular markers. Twelve fungal and ten bacterial antagonists
were tested against M. phaseolina under laboratory. The mode of antagonism
against M. phaseolina was studied under both in vitro and in vivo conditions.
Five bioagents viz., T. viride (Tv-BKN), T. harzianum (Th-BKN), T. atroviride (Ta-
15), P. fluorescens (Pf-18) and B. subtilis (Bs-15) were used alone and in
combinations as seed treatment (ST), soil application (SA) and both ST+SA to
evaluate their efficacy against dry root rot of clusterbean in green house
condition. The induction of defense related enzymes i.e. PO, PPO, PAL,
chitinase, β-1,3-glucanase, protease and the total soluble protein content in root
tissues of two test clusterbean genotypes was studied under field conditions.
Disease control efficacy of three selected bioagents used as seed treatment (ST)
and soil application (SA) along with or without organic amendment and two
botanicals was evaluated against Macrophomina dry root rot of clusterbean
under field conditions. Plant growth parameters viz., disease incidence, root and
shoot length and grain yield at harvest were recorded along with the population
dynamics of M. phaseolina and three bioagents in rhizosphere soil was
recorded.
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