Chemoprofiling and biological screening of Rhus parviflora collected from Uttarakhand
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Date
2022-08
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G. B. Pant University of Agriculture and Technology, Pantnagar
Abstract
The present study was conducted on phytochemical analysis and biological activities of leaf and stem extracts of Rhus parviflora collected from Simli, Chamoli (Uttarakhand). Extracts were prepared separately by soxhlet extraction method using three different solvents viz. hexane, methanol and chloroform. The methanolic extract showed highest percent yields followed by chloroform and hexane. The extracts were screened for various biological activities such as antioxidant, antibacterial, anti-inflammatory and α-amylase inhibition activities. Antioxidant activity of the extracts were checked with some standard methods viz. DPPH (2,2-diphenyl-1-picrylhydrazyl), FRAP (Ferric ion reducing antioxidant potential), metal chelating methods and hydroxyl radical scavenging methods. The highest DPPH activity was shown by R. parviflora leaf methanol (RPLM) extract having IC50 value 371.61±3.43 μg/mL. Stem methanol (RPSM) extract exhibited maximum FRAP potential with value of 23.10±0.031 μmole/mL. Highest metal chelating activity is shown by plant extract having IC50 value 913.40±0.38 μg/mL. RPLM extract having IC50 value 167.6±2.94 μg/mL showed maximum peroxide radical scavenging activity. The amount of total phenolics was found to be maximum in RPLM extract (95.74±0.289 GAE mg/g). Strongest anti-inflammatory was observed in RPLM with an IC50 value 296.37±1.82 μg/mL. Highest α- amylase inhibition activity was reported in RPLM with IC50 value 348.72±2.20 μg/mL. Leaf and stem extracts of plant R. parviflora showed effective antimicrobial activity against gram positive bacteria (B. subtilis and S. aureus) and gram negative bacteria (E. coli and S. typhi) and the strongest antimicrobial was showed by methanolic plant extracts. GC-MS of those extracts have been carried out which were having good biological activities to reveal the phytoconstituents present in it. The major constituents were identified are olean-12-en- 3, beta-ol acetate (32.06%), Guanosine (23.53%), Desulphosinigrin (17.87%), Phenol, 3-(10Z) 10-heptadecen-1-yl (14.4%), gamma-sitosterol (12.94%), vitamin E (11.76%), β-amyrin (11.69%), 24-Norursa-3,12-diene (10.71%), chalcone (7.03%), hexacosane (7.64%) and lupeol acetate (6.14%). Molecular Docking was performed by the Salmonella typhi OmpF complex (PDB Id- 4KR4) with the major phytoconstituents identified by GC-MS analysis. On the basis of the above results, it can be concluded that Rhus parviflora plant is rich in phytochemicals and may be used as an antioxidants, anti-inflammatory, antidiabetic and antimicrobial agent.