Detection of Hepatozoan canis in Dogs from Namakkal Region of Tamilnadu by Conventional and Molecular Approach
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Int. J. Curr. Microbiology and Applied Sciences
Abstract
A total of 150 dogs brought to Teaching Veterinary Clinical Complex (TCCC),
Veterinary College and Research Institute, Namakkal, from September, 2015 to
April, 2016 were screened for the haemoparasite, H. canis by blood smear
examination and polymerase chain reaction which revealed a positivity of 0.66
and 5.33 per cent, respectively. The PCR was found to have a high sensitivity
(100.0%) and specificity (91.27%) when compared with microscopic examination.
The target 18S rRNA of H. canis identified was found to possess a 100.0 per cent
homology with the H. canis of dogs in Hungary, Italy, Japan, North Africa,
Pakistan, Portugal and Thailand. Rhipicephalus sanguineus was the tick vector
identified from the positive cases and the males (6.48%), cross breed dogs (10.0%)
and the age group of >4 years (9.09%) were found to be highly susceptible.
Description
Keywords
Veterinary Science, Veterinary Preventive Medicine