EFFICACY OF THE SOME PROGRAMMED BREEDING PROTOCOLS ON FERTILITY FOLLOWING TIMED AI IN DAIRY COWS IN HIMACHAL PRADESH
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Date
2016-07-20
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CSKHPKV, Palampur
Abstract
ABSTRACT
The work was conducted at the University dairy farm and in nearby field
institutions. In all, 201 cows were divided into three treatments viz; Ovsynch,
Heatsynch and Doublesynch synchronization protocols and one control groups.
Treatment was started in all the cyclic on day 6 of estrous cycle after the observed
heat.
In the ovsynch protocol group, cows were injected GnRH-PG-GnRH
hormones on day 0, 7 and 9, respectively. Similarly, in the Doublesynch protocol
group PG-GnRH-PG-GnRH were given on days -2, 0, 7 and 9 and in the Heatsynch
protocol group cows GnRH-PG-EB was injected on day 0, 7 and 8, respectively. All
these cows were inseminated on day 10 and 11. Control cows did not receive any
treatment and were inseminated on the day of observed heat. Ovsynch protocol
improved conception in cows when the protocol was initiated on day 6 of estrous
cycle. The highest conception rate was recorded in the cows synchronized with
Ovsynch protocol (47.91%) followed by Doublesynch (41.66%), Heatsynch (36.17%)
and control (37.14%) groups.
Blood was collected from 66 cows of different treatments and control groups
for the analysis of plasma P4, T3, T4, TSH using Radioimmunoassay procedure.
Four blood samples each were collected on days 0, 7, 8/9 (day 8 in Heatsynch only
and day 9 in Ovsynch and Doublesynch only) and 10. There was no difference in
hormonal concentrations between different protocols.
Transrectal ultrasonographic examination of the ovaries was done in the cows
to study the follicular dynamics and to establish a relationship between the follicle
size and pregnancy status. There was a significant relation between follicular size
at AI and pregnancy rate in cows.
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