In-vitro culture as a diagnostic aid to detect Blastocystis infection in food animals
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
TANUVAS
Abstract
Blastocystis is a unicellular, anaerobic, eukaryotic, polymorphic protist, which lives
in the intestinal tract of various hosts. The present study was conducted with an aim to
use in-vitro culture using Jones’ medium to diagnose Blastocystis infection. Among the
70 gut contents of different animals cultured in Jones’ medium, 50 were positive, which
included 15 microscopically negative samples. In Jones’ medium, organisms appeared
generally ovoid or rounded in profile of different sizes, being only occasionally slightly
irregular in outline. Vacuolar and granular forms were the most predominant forms
found in in-vitro culture of Jones’ medium. The occurrence of granular forms was found
to increase in older culture. Amoeboid form was rarely observed. In culture, stages
of binary fission, peanut shaped, polymorphic budding stages were recorded. In vitro
culture in Jones’ medium can be a useful diagnostic tool as well as a means to obtain
pure cultures of Blastocystis.
Description
TNV_JVP_2017_32(1)17-21
Keywords
Veterinary Science