Isolation and characterization of disease causing agents in freshwater prawn (Macrobrachium rosenbergii)
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Date
2006
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Publisher
CCSHAU
Abstract
A variety of diseases have been observed in larval, juvenile and adult
freshwater prawns viz. as tail rot, larval mid cycle, bacterial necrosis, black
spot disease, body cramp, black gills, and vibriosis in three selected fish
farms (Ladwa, Mirka farm, Hisar and Charanjeet farm, Hansi) from July to
December,2005. Tail rot disease was found in all the months during the
course of work. The emergence of disease is actually the result of the
interaction between abiotic and biotic factors. Keeping in view the above
facts, the present investigations were carried out to identify disease causing
pathogens in freshwater prawn, M. rosenbergii. A number of biochemical
tests were performed on sugars, alcohol and amino acid etc were carried out
for the identification of bacterial pathogens. Eighteen gram - negative rods
(Aeromonas hydrophila, Enterobacter aerogenes, Escherichia coli,
Klebsiella oxytoca, Kl. pneumoniae subsp. aerogenes, Kl. pneumoniae
subsp. ozaenae, Citrobacter freundii, C. koseri, Pseudomonas aeruginosa,
P. alcaligenes, P. putida, P. stutzeri ,P. fluorescens,Vibrio alginolyticus, V.
splendidus, V. logei, V. parahaemolyticus, V. diazotrophicus, V. fluvialis)
three gram positive - bacilli (Lactobacillus casei, L. brevis, L. lactis) and five
gram - positive cocci (Micrococcus luteus1, M. varians, Cellobiosococcus
sciuri, Staphylococcus aureus, Streptococcus grp Q1) were found
associated with these diseases. Fluctuations in optimum range of
physicochemical parameters provide optimum environment for the growth of
bacterial pathogens which resulted the prawns in stress conditions and
hence, causes diseases.