ROLE OF HELICASE IN STRESS TOLERANCE: AN ANALYSIS THROUGH TRANSGENIC APPROACH IN CROPS
| dc.contributor.advisor | M, UDAYAKUMAR | |
| dc.contributor.author | MAHESH | |
| dc.date.accessioned | 2016-10-24T10:26:57Z | |
| dc.date.available | 2016-10-24T10:26:57Z | |
| dc.date.issued | 28-07-14 | |
| dc.description.abstract | Plants are often exposed to diverse environmental cues, among which the abiotic stressor, drought is the major factor which limits plant growth and productivity. Plants have evolved various mechanisms and traits to maintain intrinsic cellular tolerance and improved water relations under drought. Cellular level tolerance is achieved by several mechanisms and amongst them protein turnover which is affected under stress, is the most relevant trait to be improved. From this context, stress responsive RNA helicases which sustain translation assumes significance in sustaining protein turnover under stress. The present study was aimed to clone and characterise stress responsive RNA helicases AhRH47 and AhRH68 from stress adapted species groundnut. AhRH47 is involved in removal of secondary structures at leader sequence of mRNA. Arabidopsis transgenic plants constitutively overexpressing AhRH47 showed increased protein synthesis under stress and better tolerance under salinity and mannitol stresses, besides higher survival and biomass production under moisture stress. A partial sequence of AhRH68 was cloned from groundnut and it showed high expression under salinity stress. The predicted protein structure suggested that it associates with spliceosome and plays a role in removing secondary structures in premRNA. AhRH68 is highly homologous to p68 (Pisum sativum RNA helicase 68). Groundnut transgenics expressing p68 showed intrinsic cellular tolerance to ethrel induced senescence, salinity, oxidative and simulated moisture stresses. Further, in T3 generation, transgenics showed superior survival and recovery under temperature stress, severe moisture stress and oxidative stress at whole plant level. Productivity was one of the criteria apart from intrinsic tolerance for selection of promising lines. The promising transgenic lines advanced to T4 generation showed 33% higher productivity over wild type. From these findings, it suggests that selected p68 overexpressing groundnut transgenic plants are highly productive. Hence, AhRH47 and p68 RNA helicase are potential candidate genes to improve adaptation to abiotic stress. | en_US |
| dc.identifier.other | TH-10877 | |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/81443 | |
| dc.language.iso | en | en_US |
| dc.publisher | University of Agricultural Sciences GKVK, Bangalore | en_US |
| dc.sub | Crop Physiology | en_US |
| dc.subject | --- | en_US |
| dc.title | ROLE OF HELICASE IN STRESS TOLERANCE: AN ANALYSIS THROUGH TRANSGENIC APPROACH IN CROPS | en_US |
| dc.type | Thesis | en_US |