CHARACTERIZATION OF MAIZE BANDED LEAF AND SHEATH BLIGHT PATHOGEN AND ITS MANAGEMENT

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dc.contributor.advisorPRASANNA KUMARI, V.
dc.contributor.authorGUNASRI, REDDI
dc.date.accessioned2022-08-11T08:52:16Z
dc.date.available2022-08-11T08:52:16Z
dc.date.issued2022-08-11
dc.descriptionCHARACTERIZATION OF MAIZE BANDED LEAF AND SHEATH BLIGHT PATHOGEN AND ITS MANAGEMENTen_US
dc.description.abstractIn the present investigation on “Characterization of Maize Banded Leaf and Sheath Blight Pathogen and its Management”, survey during rabi, 2018-19 in five major maize growing districts of A.P. revealed BLSB to an extent of 61.35% (West Godavari), 58.05% (East Godavari), 46.87% (Vizianagaram), 25.85% (Guntur) and 23.02 % (Kurnool). In vitro studies on cultural and morphological variability of 18 R. solani f. sp. sasakii isolates obtained from maize growing areas of A.P. indicated that colony colour was initially white later varied to light yellowish brown (6/3-2.5 Y), light brown (6/3-7.5 YR), pale brown (6/3 10YR) and brownish yellow (6/6 10YR). Of the isolates RWGB-01, REGP-05, REGJ-07, REGK-08, RVZM-10, RVZB-11 were found superior in their radial growth (9.00 cm) with high growth rate (0.75 mm h-1). Four different growth patterns with profuse aerial mycelium were observed. Hyphal width among isolates ranged from 6.69 to 8.66 μm (RWGB-01). The sclerotia size varied from 0.93 (RKLC-18) to 1.86 mm (RWGB-01 and RWGN-03) and sclerotia weight ranged from 5.97 (RKLR-17) to 61.32 mg (RGUG-13). Time taken to form sclerotia varied from five to eight days with wide variation in colour. Three different types of sclerotial distribution were observed, i.e., aerial, surface and, surface and aerial. Five Different types of patterns of arrangement of sclerotial bodies in culture plates were observed. Sclerotia number ranged from 10 to 122. Two types of sclerotia textures i.e., rough and smooth were observed. xvii The incubation period varied from 72 to 120 h. Among all the isolates, REGP-05 isolate was found highly virulent with low incubation period (72 h) resulting in large lesions (22.43 cm) and highest PDI (49.33%). Molecular variability was determined for the 18 test isolates using four RAPD and two ISSR primers which resulted in a total of 108 bands with polymorphism having band size ranged from 300 to 3000 bp. Dendrogram obtained had two clusters (I and II), cluster I consisted of single isolate (RGUG-13) and cluster II consisted of remaining 17 isolates. Cluster I had 66% similarity with cluster II. The average PIC value (0.27) was similar to both the primers whereas average Rp value was high for ISSR primers (6.21) when compared to RAPD primers (5.92). Isozyme variability in esterase and peroxidase enzymes was determined for the R. solani f. sp. sasakii isolates. A total of 18 bands with Rm values ranging from 0.24 to 0.54 were obtained. Dendrogram obtained had three clusters (A, B and C). Cluster A consisting of two isolates RWGN-03 and RWGD-04 while cluster B consisting of four isolates (RWGK-02, REGJ-07, REGK-08 and RVZS-09). Cluster C was comprised of remaining 12 isolates. Cluster A was 60% similar to cluster B, C and cluster B was 75% similar to cluster C. Under in vitro conditions Karanj leaf extract @ 15% resulted in the maximum mycelial inhibition of pathogen (3.18 cm) followed by Karanj leaf extract @ 10% (4.12 cm) and Nerium leaf extract @ 5% (4.50 cm). Among all the tested cow based natural products (CBNP), Panchagavya was significantly superior in inhibiting the radial growth of the pathogen at all the concentrations, i.e., 5%, 10%, 15% (0.00 cm) and was on par with the cow urine @ 10, 15% (0.00 cm) and cow dung + cow urine @ 15% (0.00 cm). Pooled data analysis of both the seasons (rabi 2018-19 and 2019-20) revealed efficacy of Karanj leaf extract @ 15%+Panchagavya @ 5% in controlling the disease even two weeks after second spray (71 DAS) with low PDI (35.77%) and minimum AUDPC (670.60) compared to unsprayed check (1137.16). Treatment Karanj leaf extract @ 15%+Panchagavya @ 5% recoded maximum number of cobs/m2 (9), test weight (22.83 g), yield (7469.14 Kg ha-1) and B: C ratio (2.55) as against the control (1.43).en_US
dc.identifier.otherD6242
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810186226
dc.keywordsCHARACTERIZATION OF MAIZE BANDED LEAF AND SHEATH BLIGHT PATHOGEN AND ITS MANAGEMENTen_US
dc.language.isoEnglishen_US
dc.pages188en_US
dc.publishergunturen_US
dc.subPlant Pathologyen_US
dc.themeCHARACTERIZATION OF MAIZE BANDED LEAF AND SHEATH BLIGHT PATHOGEN AND ITS MANAGEMENTen_US
dc.these.typePh.Den_US
dc.titleCHARACTERIZATION OF MAIZE BANDED LEAF AND SHEATH BLIGHT PATHOGEN AND ITS MANAGEMENTen_US
dc.typeThesisen_US
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