Morpho-molecular characterization of Hazelnut (Corylus avellana L.)
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Date
2021-12-04
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College of Horticulture and Forestry Neri, Hamirpur (H.P.)
Abstract
The present investigation entitled, “Morpho-molecular characterization of Hazelnut
(Corylus avellana L.)” was carried out at Horticulture Research Station Seobagh, Kullu (HP) during
2019 to 2021.A significant and wide range of variation was observed among genotypes for various
quantitative characters. In variability study, total nut thickness had maximum phenotypic coefficient
of variation (PCV) and genotypic coefficient of variation (GCV) followed by nut length (mm).
High heritability (>60%) was recorded for all the characters except leaf length (mm), kernel
thickness (mm) and kernel weight (g). High genetic gain was recorded in leaf width (cm), nut
length (mm), nut width (mm), nut thickness (mm), nut weight (g), kernel length and width (mm).
Ennis recorded maximum nut length, nut width, thickness, weight and kernel length. Phenotypic and
genotypic correlation coefficients showed that nut weight had positive and significant association
with kernel weight. The path coefficient analysis has shown that the kernel weight had maximum
positive direct effect on nut weight followed by kernel thickness. The maximum negative direct
effect of nut length followed by kernel thickness was observed on kernel weight. Principle
component analysis showed total variability of 95.33% with first five components i.e., leaf length, leaf
width, nut length, width and thickness. Leaf length contributed maximum variability of 49.15%
followed by leaf width with a value of 22.14%. Morphological clustering formed three main clusters,
first including Ennis variety followed by second cluster comprising Fertile de Coutard, Merveille de
Bollwiller and third cluster included remaining four varieties. Genetic diversity among the hazelnut
cultivars was estimated by 47 RAPD and 10 ISSR markers. 17 primers out of total of forty seven
primers yielded scorable bands. A total of 91 bands were amplified in all the eight cultivars and
83.5% polymorphism was obtained with RAPD markers whereas, 48 amplified bands were recorded
with 85.4% polymorphism with ISSR markers. This showed the high level of polymorphism present
in eight hazelnut cultivars under this study. The similarity coefficient value ranged from 0.000 to
0.937 for RAPD and 0.000 to 0.492 in case of ISSR markers. Dendrogram divides all the varieties in
one cluster and Ennis was separated as a singlet for both RAPD and ISSR markers. Further, subclusters
formed comprised Italian varieties separately and French varieties separately.