DEVELOPMENT AND VALIDATION OF CORE COLLECTIONS OF DOLICHOS BEAN (Lablab purpureus L. Sweet) GERMPLASM
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Date
2013-07-23
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University of Agricultural Sciences GKVK, Bangalore
Abstract
Development of a reduced representative set of a large base
collection enable its enhanced use in crop improvement. Considering the
size of current Dolichos bean germplasm (648 accessions) being
maintained at AICRP on pigeonpea, ZARS, UAS, GKVK, Bangalore is
unwieldy for management, characterization and evaluation and hence
increased use in breeding Dolichos bean, an attempt was made to
develop a core set from Dolichos bean germplasm. 644 germplasm
accessions along with four check varieties were characterized for 21
qualitative traits and evaluated for 20 quantitative traits following
Augmented design during Kharif 2012 at ZARS, UAS, GKVK, Bangalore.
Substantial polymorphism for qualitative traits and genetic variability for
quantitative traits in the germplasm were evident.
A total of 10 core sets were developed using 20 quantitative traits
data following standard stratified sampling and heuristic strategiesbased
approaches. Qualitative traits-based statistics such as chi-square
test and ‘class coverage’, and ‘Shannon-Weaver diversity index’ were
used as validation criteria to examine homogeneity and retention of
qualitative traits-based classes, and polymorphism diversity, respectively
in the core sets to assess their representativeness. Quantitative traitsbased
univariate statistics -mean and variance and standardized
multivariate statistics such as SMD%, CR%, VD% and VR% were used to
assess the representativeness of core sets. Different core sets were
representative of the base collection based on different validation criteria.
Heuristic approach-based core set of 10 per cent size was identified as
the best representative of the base collection. This core set is suggested
as a priority resource for use in breeding Dolichos bean.
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