Effect of thermal regimes on growth and immuno-biochemical responses of golden mahseer (Tor putitora)
Loading...
Date
2018-07
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
The present study was carried out to elucidate the effect of different thermal regimes on growth and immuno-biochemical responses of golden mahseer (Tor putitora) fingerlings. Initially, around 200 golden mahseer fingerlings were collected and acclimatized at 20 °C for 10 days. Then 144 healthy fingerlings (9.0 ± 0.23 cm length and 8.36 ± 0.45 g weight) were randomly distributed into four experimental groups (Ambient temperature; ABT-control, ABT+3 C̊ , ABT+ 6 C̊ , ABT+ 9 ̊C) in triplicate rectangular FRP tanks. Fishes were fed with formulated pelleted feed twice daily (10.00 am and 5.00 pm) @ 2 % body weight. Growth and water quality parameters (temperature, pH, DO, free CO2, alkalinity) were analysed during the study period. At the end of the experiment, immunological parameters (i.e. glucose, haemoglobin and haemetocrit, lysozyme, myeloperoxidase) and stress biomarkers (i.e. growth hormone, lipid peroxidation, antioxidative potential, superoxide dismutase, catalase and glutathione s-transferase) were analysed. All the water quality parameters were suitable for golden mahseer fingerlings. Result showed significantly higher growth, specific growth rate and growth hormone levels observed at ABT+ 6 ̊C group compared to other group. Lower FCR was obtained at temperature 19.52 ± 0.71 °C. Hepato-somatic index was found maximum at ABT while viscera-somatic index was maximum in ABT+ 3 C̊ group. Result on length-weight relationship was shown allometric growth in golden mahseer fingerlings. Haemoglobin and haemetocrit values significantly decreased with increase in temperature. Myeloperoxidase activity was found higher upto temperature ABT+ 3 C̊ and its activity decreased in ABT+ 6 C̊ and ABT+ 9 C̊ groups. Highest lysozyme activity and TBARS levels were evident in ABT+3 C̊ group. Anti-oxidant potential was found highest at ABT group and decreased gradually with increase in temperature. The response of anti-oxidative enzymes was tissue specific and significantly different at different thermal regimes. Present study reveals different phenotypic and immuno-biochemical modulations of golden mahseer fingerlings in response to different temperature regimes which can be used for its better management and propagation.
Description
Keywords
null