DETECTION OF GENETIC VARIABILITY AMONG CHILLI GERMPLASM USING MOLECULAR MARKERS

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dc.contributor.advisorSIVARAMAKRISHNAN, S
dc.contributor.authorRAVI, K
dc.date.accessioned2016-08-17T13:55:47Z
dc.date.available2016-08-17T13:55:47Z
dc.date.issued2006
dc.description.abstractAssessment of genetic diversity is important for crop improvement and also efficient management and protection of germplasm resources. Purposeful plant breeding rests on the wise use of genetic resources. Chilli is an important spice crop, which is consumed as spice and also as a vegetable. It contains oleoresins like capsaicin and capsanthin, which impart pungency and colour to the fruits. The present study was taken up with an objective to assess inter accession diversity among 140 chilli germplasm accessions belonging to the species C. annum L. collected from different origin/sources. 30 SSR marker primers were selected for this study. All the marker primers were initially evaluated for annealing temperature using a gradient PCR technique. Among the 30 marker primers screened for polymorphism 6 marker primers were found to be polymorphic and these 6 marker primers were used to analyze the 140 chilli germplasm accessions. The maximum number of alleles detected was five with two primers and a least number of two with one primer. Among the six primers one primer exhibited a large number of null alleles, apart from this primer ten accessions showed null alleles with different marker primers. So these were excluded from cluster analysis. At these six loci a total of 21 alleles were detected with an average of 3.5 alleles per locus. The pair wise similarity based on Dice coefficient for all the 130 accessions ranged from 0.00 to 1.00. The cophenetic correlation coefficient (r) computed between the observed distances and dendrogram was 0.66 which indicates a good fit between observed distances and dendrogram. The clustering revealed that the overall grouping of accessions is based on geographical association with few exceptions. Further many accessions were found to be indistinguishable with a similarity coefficient of one. In our analysis using 6 SSR marker primers about 32% of genetic divergence was explained by the first 2 ordinates of the PCoA. The cophenetic correlation coefficient (r) between observed distances and the PCoA was 0.81. The MDS plot showed the 130 accessions to be almost randomly spread over two-dimensional Euclidian space with a cophenetic correlation coefficient of 0.6en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/72762
dc.language.isoenen_US
dc.publisherACHARYA N.G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABADen_US
dc.relation.ispartofseriesD7890;
dc.subAgricultural Biotechnology
dc.subjectDETECTION, GENETIC, VARIABILITY, AMONG, CHILLI, GERMPLASM, USING, MOLECULAR, MARKERSen_US
dc.these.typeM.Sc
dc.titleDETECTION OF GENETIC VARIABILITY AMONG CHILLI GERMPLASM USING MOLECULAR MARKERSen_US
dc.typeThesisen_US
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