MOLECULAR, PHYSIO-BIOCHEMICAL ANALYSIS ON SEED LONGEVITY AND OPTIMIZATION OF SEED RATE COMPENSATION IN SOYBEAN [Glycine max (L.) Merrill]
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Date
2022-12-29
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University of Agricultural Sciences, Bangalore
Abstract
The laboratory studies were conducted with 20 soybean genotypes for evaluating the physiological and biochemical changes on seed longevity. 5 different testa coloured soybean genotypes had used for the experiment. Among them, Black coat seeds had showed resistant for seed longevity and yellow coloured genotypes exhibited susceptibility for seed longevity. Among the seed amelioration treatments, seed priming with tocopherol (1%) increases repair and re-synthesizing ability in low vigour seeds (yellow coloured seeds) followed by salicylic acid (1%) and ascorbic acid (1%). 20 soybean genotypes were subjected to molecular phenotyping with SSR markers like Satt281, Satt513, Satt371 produces three alleles viz., Satt281220, Satt513200 and Satt371180 for good storer genotypes, Satt281200, Satt371120 and Satt513140 for poor storers and Satt281210, Satt513150, Satt371160 for intermediate storers. Therefore, these markers may be good candidate markers for identifying marker linked with storability
and seed coat colour. The field experiment was conducted to assess the effect of seed rate compensation, among the treatments high vigour seeds with normal seed rate (62.5 kg /ha) obtained the highest plant height, seed yield per plant and seed yield per hectare (45.92 cm, 9.60 g and 21.11 q respectively) followed by aged seeds of 65 per cent germination with compensated seed rate (67.3 kg / ha). The lowest plant height, seed yield per plant and seed yield per hectare (35.55 cm, 7.72 g and 9.99 q) were noticed in aged seeds of 50 per cent germination with compensated seed rate (87.5 kg /ha).