MICROBIOME OF RICE AS INFLUENCED BY CYANOBACTERIAL INOCULATION
| dc.contributor.advisor | Radha Prasanna | |
| dc.contributor.author | HIMANI PRIYA | |
| dc.date.accessioned | 2017-06-12T07:07:57Z | |
| dc.date.available | 2017-06-12T07:07:57Z | |
| dc.date.issued | 2014 | |
| dc.description | t-9021 | en_US |
| dc.description.abstract | Plants are full of microbes and phenotypic expression of most plants in nature is the product of highly co-regulated expression of genes both from plants and microbes. The present investigation was undertaken to analyse the effect of cyanobacterial inoculation on the structure and function of microbial communities in soil and the rice microbiome, using a combination of culturable and culture-independent approaches. The cultural bacterial diversity of the rice plant microbiome (microbial communities in roots and shoots) was investigated in the presence and absence of cyanobacterial inoculation in rice seedlings, under controlled conditions in water agar using rice cultivar cv. Pusa Sugandh 5 (Pusa 2511). Population densities ranging from 106 -109 CFU/ml of culturable microorganisms were recorded in the root and shoot tissues in different types of nutrient media. Five and three isolates exhibited nitrogen fixation and phosphorus solubilisation, respectively. Sixteen morphotypes were selected for profiling of fatty acid methyl esters (FAME) and 16S rDNA sequencing, which showed that about 50% of the isolates belonged to the Family Bacillaceae of the Phylum Firmicutes. A significant enhancement in plant growth and physiological attributes were recorded which was complemented by SEM observations revealing an enhancement in rod-shaped cells in both root and shoot tissues of Calothrix inoculated plants, along with the presence of short 2-3 celled filaments, presumably of Calothrix sp. In order to understand the interactions of soil and plant microbiome, with or without cyanobacterial inoculation, a pot experiment, using sterile and unsterile soil was carried out under the controlled conditions of the National Phytotron Facility, IARI, New Delhi. Culture-independent, microbial community-based approaches such as phospholipid fatty acid (PLFA) and denaturing gradient gel electrophoresis (DGGE) profiling, complemented by evaluation of soil nutrient status and microbiological parameters were employed. Principal component analysis of these PLFA profiles showed that inoculation with Calothrix brought about distinct temporal changes in the concentrations of individual PLFA(s) and microbial guilds. DGGE based studies illustrated the significant role of cyanobacteria in modulating the microbiome of rice. It can be concluded that positive effect of cyanobacterial inoculation on the rice plant growth is probably due to its role in maintaining the “beneficial core microbiome of rice." | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810019448 | |
| dc.keywords | MICROBIOME OF RICE AS INFLUENCED BY CYANOBACTERIAL INOCULATION | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Division of Microbiology Indian Agricultural Research Institute New Delhi | en_US |
| dc.sub | Microbiology | en_US |
| dc.subject | null | en_US |
| dc.theme | MICROBIOME OF RICE AS INFLUENCED BY CYANOBACTERIAL INOCULATION | en_US |
| dc.these.type | M.Sc | en_US |
| dc.title | MICROBIOME OF RICE AS INFLUENCED BY CYANOBACTERIAL INOCULATION | en_US |
| dc.type | Thesis | en_US |