Validation of anthracnose [Colletotrichum lindemuthianum (Sacc. & Magnus) Scribner] resistance specific micro satellite markers in French bean (Phaseolus vulgaris L.) parental lines and development of trait specific mapping population
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Date
2014-08-15
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University of Agricultural Sciences, Bangalore
Abstract
French bean is one of the most important legume, next to chickpea grown extensively in Asian countries. It is a popular vegetable and dry seeds are used as pulse. It is rich in protein, calcium and vitamins. It is a short duration crop grown in Kharif and Rabi seasons. It is prone to several abiotic and biotic stresses among them the fungal disease caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Scribner. is the major one causing severe yield loss up to 40-50%. Thus a study on development of mapping populations between the two popular French bean varieties Jwala and Kanchana (susceptible varieties) and three resistant lines (L-line, D-line, EC500641) was carried out. The F1 and F2 populations were developed from the above parental lines, further they were screened using twelve SSR markers, out of which three (PVatcc001, PH7B3 and PVag001) SSR markers were polymorphic for the parental lines. Thus they were used to confirm the true F1 hybrids and F2 populations resistant to anthracnose and the markers could differentiate susceptible and resistant populations. Thus the above three markers can be effectively used in studies to confirm mapping populations developed for
anthracnose resistance.
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