BIO-MANAGEMENT OF BLAST OF FINGER MILLET (RAGI) CAUSED BY Pyricularia grisea (Cke.) Sacc.
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Date
2016-07-26
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Abstract
Finger millet is an important millet crop of southern Karnataka, affected by blast
disease that causes yield loss up to 50 to 60 per cent. A total of 35 bacterial and six fungal
antagonistic organisms isolated from finger millet phyllosphere were identified as species
of Pseudomonas, Bacillus and Trichoderma based on the cultural, morphological and
biochemical tests. In vitro evaluation of phyllosphere microflora against P. grisea
revealed that Bacillus isolates B13, B3, RBs1, B7 and Pseudomonas isolates P2, P17,
P22, RPf1 and T. harzianum (Th3) recorded maximum inhibition of 66.79, 66.41, 66.29,
59.35, 62.24, 52.16, 50.16, 50.09 and 56.60 per cent respectively. Bacillus isolate B13
found potential against P. grisea which was further identified as Bacillus
amyloliquefaciens based on 16S rRNA sequence. and these isolates were tested for their
efficacy under pot culture. Foliar spray of P. fluorescens (RPf1) recorded minimum PDI
of leaf (30.46), neck (1.59) and finger (3.12) blast besides maximum plant growth
promotion in-terms of shoot (106.20 cm) and root length (42.83 cm) which was on par
with B. amyloliquefaciens and T. harzianum. Further, field evaluation of these bioagents
revealed that Seed treatment + Seedling dip + Foliar spray with P. fluorescens RPf1
recorded minimum PDI of leaf (19.49), neck (1.42) and finger blast (1.26) with
maximum growth promotion i.e. shoot length (99.10 cm), fresh shoot weight (45.33 g),
grain yield (1900 kg ha-1) and straw yield (5375 kg ha-1) followed by B.
amyloliquefaceins confirming usage of bioagents in the management of finger millet
blast.
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