IDENTIFICATION AND BIO-MANAGEMENT OF ROOT-KNOT NEMATODE (Meloidogyne enterolobii) IN GUAVA (Psidium guajava L.)
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Date
2023-04-04
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University of Agricultural Sciences, Bangalore
Abstract
Meloidogyne enterolobii is an emerging and most serious pest of guava in Southern India. Biological control method is an effective strategy in management of root knot nematodes ensuring soil fertility and environmental health. The goal of our study is to
evaluate the distribution and severity of M. enterolobii on guava in Southern Karnataka and find an effective combination of bioagents for root knot nematode (RKN) management. Survey conducted in a total of 10 guava orchards from four districts of Southern Karnataka, the major guava cultivating areas revealed the incidence of M. enterolobii in all the places. Visual evaluation of infested roots by galling index (RKI) recorded the highest distribution and severity of RKN in Ramanagara district (RKI-4.00) followed by Bengaluru (RKI-3.00) and Bengaluru rural (RKI-2.00). The identity of the nematode isolates was confirmed by a
combination of morphological and molecular methods. The cuticular modifications at the posterior region of the female nematode were consistent with the perineal patterns of M. enterolobii. Further, molecular characterization of isolate GBN-1, by sequencing of 18SrRNA region confirmed the nematode as M. enterolobii. Among various combination of bioagents tested under field conditions revealed that the treatment T5 [Trichoderma asperellum+Bacillus subtilis+neemcake+African Marigold (30mL+30mL+100g/plant)]
imposed at monthly interval was effective in reducing the nematode population in soil (746.67/200 cc) and roots (149.33/5g root), maximum reduction of galls (13.67/5g root), egg masses (17.67/5g root) and over control (1248.11/200 cc, 284.33/5g root, 36.00/5g
root, 41.33/5g root) of the nematodes respectively.