Detection and differentiation of pathogens of Mycobacterium tuberculosis complex in bovines

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dc.contributor.advisorFilia, Gursimran
dc.contributor.authorGill, Amandeep Kaur
dc.date.accessioned2019-10-16T09:17:17Z
dc.date.available2019-10-16T09:17:17Z
dc.date.issued2017
dc.description.abstractBovine tuberculosis, chronic disease of animals, caused by species of Mycobacterium tuberculosis complex (MTC) remains a potential threat to animals as well as humans. Differentiation of the species of MTC is required for epidemiological and diagnostic purpose. The present study evaluated the presence of different species of MTC in bovines using gyrB-restriction fragment length polymorphism analysis. In this study, blood and milk samples from 50 milch animals which were positive reactors of comparative intradermal tuberculin test (CITT) were collected. Screening of MTC was done by IS6110-PCR using specific primers INS1/INS2 specific for MTC. Four (8%) animal were positive for MTC by IS6110-PCR. The positive samples were further confirmed by primers specific for gyrB gene. The amplified product of the gyrB-PCR was digested by RsaI and SacII enzymes and restriction fragment length polymorphism was carried out. The result of gyrB-RFLP revealed two animals positive for M. bovis and two for M. tuberculosis. Thus, gyrB-RFLP could be used as an additional tool in differential diagnosis of mycobacterial diseases caused by different species of MTC.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810131466
dc.keywordsMycobacterium tuberculosis complex, Mycobacterium bovis, Mycobacterium tuberculosis, Comparative Intradermal Tuberculin Test, Polymerase Chain Reaction, Restriction Fragment Length Polymorphismen_US
dc.language.isoenen_US
dc.pages59en_US
dc.publisherGuru Angad Dev Veterinary and Animal Sciences University, Ludhianaen_US
dc.subVeterinary Microbiologyen_US
dc.subjectnullen_US
dc.themeDetection and differentiation of pathogens of Mycobacterium tuberculosis complex in bovinesen_US
dc.these.typeM.V.Sc.en_US
dc.titleDetection and differentiation of pathogens of Mycobacterium tuberculosis complex in bovinesen_US
dc.typeThesisen_US
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