Detection and differentiation of pathogens of Mycobacterium tuberculosis complex in bovines
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Date
2017
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Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana
Abstract
Bovine tuberculosis, chronic disease of animals, caused by species of Mycobacterium tuberculosis complex (MTC) remains a potential threat to animals as well as humans. Differentiation of the species of MTC is required for epidemiological and diagnostic purpose. The present study evaluated the presence of different species of MTC in bovines using gyrB-restriction fragment length polymorphism analysis. In this study, blood and milk samples from 50 milch animals which were positive reactors of comparative intradermal tuberculin test (CITT) were collected. Screening of MTC was done by IS6110-PCR using specific primers INS1/INS2 specific for MTC. Four (8%) animal were positive for MTC by IS6110-PCR. The positive samples were further confirmed by primers specific for gyrB gene. The amplified product of the gyrB-PCR was digested by RsaI and SacII enzymes and restriction fragment length polymorphism was carried out. The result of gyrB-RFLP revealed two animals positive for M. bovis and two for M. tuberculosis. Thus, gyrB-RFLP could be used as an additional tool in differential diagnosis of mycobacterial diseases caused by different species of MTC.
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