Identification of actinobacteria from rhizosphere soils for growth promotion in tomato

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dc.contributor.advisorSaini, Raj
dc.contributor.authorMankotia, Radhika
dc.date.accessioned2023-07-18T06:18:22Z
dc.date.available2023-07-18T06:18:22Z
dc.date.issued2021-01-26
dc.description.abstractThe present investigation entitled “Identification of actinobacteria from rhizosphere soils for growth promotion in tomato” was carried out during 2019-2020 with the aim to explore native actinomycetes from rhizosphere of tomato crop grown at different locations in district Kullu and Hamirpur of Himachal Pradesh. A total of 109 actinobacterial isolates were obtained using different selective media for isolation viz., actinomycete isolation agar medium, yeast malt agar medium and starch M protein agar medium and the preliminary screening of the isolates for plant growth promoting attributes (PGP) attributes was carried out. It was found that only 88.99% of the total isolates showed phosphate (P) solubilization on Pikovskaya’s agar medium and the phosphate solubilization index varied widely from 1.11 to 6.0. All actinobacterial isolates displayed growth on nitrogen free Jensen’s medium and only 5.50% isolates exhibited siderophore production. The inhibition of fungal pathogens viz. Alternaria sp, Fusarium sp, Phytophthora sp and Sclerotinia sp., was demonstrated by 28.44%, 11.92%, 15.59% and 12.84% actinobacterial isolates, respectively and four isolates (NDF-10, HMS-4, HMM-1 and HMM-4) showed inhibition against all the phytopathogenic fungi.On the basis of initial screening, only fifteen actinobacterial isolates were further evaluated and found to exhibit high P solubilization (718.16 to 1418.16 μg/ml) in National Botanical Research Institute’s Phosphate broth with tricalcium phosphate and high indole-3-acetic acid/IAA (11.56 to 21.35 μg/ml) production. Maximum P solubilization and IAA production was observed in isolate KLK-8. The production of extracellular enzymes amylase, cellulase, gelatinase, lipase and protease varied among the isolates. All the fifteen actinobacterial isolates with filamentous morphology varied in their biochemical characteristics. Screening of actinomycete isolates for in vitro tomato seed germination and growth studies in filter paper assay revealed best performance of seeds treated with isolate KLK-8 which showed a significant increase in per cent germination, root length, shoot length and vigour index over untreated control. On the basis of 16S rRNA gene sequence analysis, three promising actinobacterial isolates KLK-8, HMM-1 and HMS-8 were identified as Streptomyces tendae strain KLK8, Streptomyces sp. strain HMM1 and Streptomyces sp. strain HMS8, respectively. The present study clearly indicates that native actinomycetes exhibit promising PGP attributes and could serve as bioinoculants to achieve more sustainable and environment friendly crop production.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810198322
dc.keywordsActinobacteria from rhizosphere, Plant growth, isolates, Tomato, Soil Scienceen_US
dc.language.isoEnglishen_US
dc.pages106+iven_US
dc.publisherCollege of Horticulture and Forestry, Dr YSP UHF, Neri, Hamirpur (H.P.)en_US
dc.subSoil Sciencesen_US
dc.themePlant growth promoting isolates and their evaluation for promoting growth in tomatoen_US
dc.these.typeM.Scen_US
dc.titleIdentification of actinobacteria from rhizosphere soils for growth promotion in tomatoen_US
dc.typeThesisen_US
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