Effect of two nanocompounds on fenugreek vigour and soil health under the influence of plant growth promoting rhizobacteria
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Date
2017-06
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G.B. Pant University of Agriculture and Technology, Pantnagar - 263145 (Uttarakhand)
Abstract
Nanotechnology has been designated as one of the most potential field for research that permits researchers to control the materials at molecular, macromolecular and atomic level. The characteristics of nanomaterials and their utility differ notably from the original compound at
macroscopic scale. Effect of two nanocompounds (Nanozeolite and Nanochitosan) on germination and vigour of fenugreek and soil health under the influence of Plant Growth Promoting Rhizobacteria was studied in a pot experiment. Two bacterial isolates (PS2 and PS10)
were selected on the basis of their plant growth promoting properties. PS2 and PS10 were characterized on the basis of molecular characters and found to have homology with Bacillus sp. on the basis of BLAST and were allotted with accession number as KX650178 and KX650179,
respectively. PS2 and PS10 produced 44.96 and 23.16 % Siderophore unit, 13.84 µg/ml and 9.29 µg/ml IAA and solubilized 2.34 µg/ml P and 2.22 µg/ml P, respectively. Enhanced average plant height, no. of leaves, leaf area and fresh weight (1.5 - 2.0 folds) over control was observed in the presence of nanocompounds + bacterial treatments. Average total chlorophyll (3.27 mg/g) and catalase activity (23.84 U/g tissue) was maximum for PS2+Nz treatment. Total soluble leaf protein was maximum (295.37mg/g fresh weight) in the PS2+Nch treatment, which was 10%
more than control. Total sugar was maximum (6.14 µg/mg dry wt.) in PS10+Nz treatment and was about 13% more than control. After 45 days of pot experiment, maximum fluorescein diacetate hydrolysis activity (2.5 fold increase) was observed in the soil under treatment of PS10+Nz. Maximum alkaline phosphatase activity i.e., 41.35 µg/g/h (11% increase) was reported in the PS10+Nz treatment whereas maximum dehydrogenase activity i.e., 3.65 µg/5g/8h (2.2 fold increase) was observed in the PS10 treatment. It can be concluded from our findings that
application of nanocompounds along with bacterial treatments improved plant-bacterial interaction, promoted plant parameters, increased microbial diversity and increased the level of soil health indicator enzymes.
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