SCREENING OF SELECTED SPECIES OF FREE RANGING WILD ANIMALS OF KERALA FOR MYCOBACTERIUM TUBERCULOSIS COMPLEX BY REAL-TIME POLYMERASE CHAIN REACTION
| dc.contributor.author | S., ASWATHY | |
| dc.date.accessioned | 2021-01-13T06:58:39Z | |
| dc.date.available | 2021-01-13T06:58:39Z | |
| dc.date.issued | 2019-08-08 | |
| dc.description.abstract | The present study was carried out to screen Mycobacterium tuberculosis complex(MTBC) in selected species of wild animals (elephants,wildboar, monkey and deer) in Kerala. The post-mortem samples from wild animals(27 numbers) from different wildlife sanctuaries in Kerala were collected and screened for MTBC infection. Molecular diagnosis was done by using real – time polymerase chain reaction targeting IS6110 and senX3-regX3 intergenic region. Conventional PCR was carried out using 16SrRNA gene and IS6110 gene.The positive PCR samples were subjected to multiplex PCR tests for plcA,recAinteinand hsp65 genes.All the samples of wild boars, deer and monkeys were negative for MTBC.A total of four elephant samples shown positive result by IS6110 gene targeted real-time PCR and three were positive for conventional PCR targeting same gene.All the samples showed negative result in senX3-regX3 intergenic region targeted real-time PCR. A total of three elephant samples showed positive result by16SrRNA targeted PCR and among them one sample shown positive result by hsp65 gene targeted PCR also. All samples were negative for plcA and rec A intein gene. Though IS6110 has been detected in the samples, none of the other tests conducted were positive for MTBC. The BLAST analysis of positive isolates points towards presence of non-tuberculous mycobacterium in these samples. Hence it is concluded that the lesions observed in these animals could be due to non-tuberculous mycobacteria | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810160011 | |
| dc.keywords | FREE RANGING WILD ANIMALS, MYCOBACTERIUM TUBERCULOSIS COMPLEX, REAL-TIME POLYMERASE CHAIN REACTION, MIRU-VNTR, RFLP, RAPD, PCR, TaqMan real-time PCR, | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 100 | en_US |
| dc.publisher | COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD | en_US |
| dc.research.problem | FREE RANGING WILD ANIMALS OF KERALA FOR MYCOBACTERIUM TUBERCULOSIS COMPLEX BY REAL-TIME POLYMERASE CHAIN REACTION | en_US |
| dc.sub | Clinical Veterinary Medicine, Ethics and Jurisprudence | en_US |
| dc.theme | SCREENING OF SELECTED SPECIES OF FREE RANGING WILD ANIMALS OF KERALA FOR MYCOBACTERIUM TUBERCULOSIS COMPLEX BY REAL-TIME POLYMERASE CHAIN REACTION | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | SCREENING OF SELECTED SPECIES OF FREE RANGING WILD ANIMALS OF KERALA FOR MYCOBACTERIUM TUBERCULOSIS COMPLEX BY REAL-TIME POLYMERASE CHAIN REACTION | en_US |
| dc.type | Thesis | en_US |