SCREENING OF SELECTED SPECIES OF FREE RANGING WILD ANIMALS OF KERALA FOR MYCOBACTERIUM TUBERCULOSIS COMPLEX BY REAL-TIME POLYMERASE CHAIN REACTION
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Date
2019-08-08
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COLLEGE OF VETERINARY AND ANIMAL SCIENCES, POOKODE WAYANAD
Abstract
The present study was carried out to screen Mycobacterium tuberculosis
complex(MTBC) in selected species of wild animals (elephants,wildboar, monkey
and deer) in Kerala. The post-mortem samples from wild animals(27 numbers) from
different wildlife sanctuaries in Kerala were collected and screened for MTBC
infection. Molecular diagnosis was done by using real – time polymerase chain
reaction targeting IS6110 and senX3-regX3 intergenic region. Conventional PCR was
carried out using 16SrRNA gene and IS6110 gene.The positive PCR samples were
subjected to multiplex PCR tests for plcA,recAinteinand hsp65 genes.All the samples
of wild boars, deer and monkeys were negative for MTBC.A total of four elephant
samples shown positive result by IS6110 gene targeted real-time PCR and three were
positive for conventional PCR targeting same gene.All the samples showed negative
result in senX3-regX3 intergenic region targeted real-time PCR. A total of three
elephant samples showed positive result by16SrRNA targeted PCR and among them
one sample shown positive result by hsp65 gene targeted PCR also. All samples were
negative for plcA and rec A intein gene. Though IS6110 has been detected in the
samples, none of the other tests conducted were positive for MTBC. The BLAST
analysis of positive isolates points towards presence of non-tuberculous
mycobacterium in these samples. Hence it is concluded that the lesions observed in
these animals could be due to non-tuberculous mycobacteria