INVITRO STUDIES ON UTERINE FLUSHING IN RELATION TO DIFFERENT PHASES OF ESTROUS CYCLE AND REPRODUCTIVE DISORDERS IN BUFFALOES
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Date
2005-03
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SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA
Abstract
ABSTRACT:
The study on invitro studies on uterine flushings in relation to different phases
of estrous cycle and reproductive disorders was carried out by procuring female
buffalo genitalia from abattoirs of Municipal Corporation of Hyderabad and Alkabeer
slaughter house private limited, Medak district. The incidence of abnormal genital tracts were 30.41 per cent among 1398 genitalia examined during the present study. Among the genital abnormalities, ovarian
abnormalities (12.09%) oviductal abnormalities (3.15%), uterine abnormalities
(10.09%), Cervical abnormalities (4.15%) and miscellaneous abnormalities (0.93%)
were observed. The total protein level in uterine flushings were high (1.80 gr %) in
endometritis condition. The albumin levels were recorded low (0.19 gr %) in
follicular cyst and no significant difference was found in the globulin and albumin
and globulin ratio. The cholesterol levels were non-significant between the phases of
estrous cycle and among the disorders. Significantly high alkaline phosphatase
activity was observed in cystic condition of the ovaries, follicular cyst (1 06.97 KAU)
and lateral cyst (1 56.62 KAU) respectively. The mineral concentration was found to be non-significnat among the phases of estrous cycle and reproductive disorders. The cellular count regarding lymphocytes was found to be significantly different among disorders without any difference between the phases of estrous cycle.
The histopathology of follicular cyst revealed thickened cystic appearance of granulosa cell layer with infiltration of inflammatory cells above granulosa cell layer. In luteal cyst hyperplasia of epithelial cells with infiltration of mononuclear cells and milid fibrosis was noticed. In hydrosalphinx mild fibrosis of interstitial connective tissue with infiltration of mononuclear cells was noticed. In endomatritis cystic
appearancl of endosmetrial glands and degeneration of endometrial granular
epithelium with infiltration of lymphocytes and neutrophils was observed.
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