In vitro antioxidant and antimicrobial activities of mustard extracts
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Date
2021-09
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CCSHAU, Hisar
Abstract
Mustard is the third most important oilseed crop after oil palm and soybean. Although mustard is widely used as edible oil and condiment, the residue left after oil extraction is popularly used as livestock and poultry feed with excellent protein quality. However, the information regarding antioxidative and antimicrobial potential of mustard seed meal is still scanty. Therefore, the objective of the present study was to extract the polyphenols from brown and yellow mustard seed meal with different solvents i.e 80% methanol, 80% ethanol, 80% acetone and 80% isopropanol. Each extract was analysed for antioxidant potential using DPPH and ABTS scavenging activity, ferric ion reducing antioxidant power (FRAP) assay, total antioxidant activity, iron chelating activity and β-Carotene content. The 80% acetone extracted maximum polyphenols followed by 80% ethanol, 80% methanol and 80% isopropanol. Brown mustard exhibited a higher amount of polyphenols as compared to yellow mustard. Yellow mustard exhibited higher antioxidant potential in terms of DPPH and ABTS scavenging activity, ferric ion reducing antioxidant power, iron chelating activity and β-Carotene content. Correlation analysis indicated a negative association between total phenolic content and DPPH, ABTS and FRAP. Antimicrobial potential was evaluated against two gram-negative (Escherichia coli and Xanthomonas campesteris), two gram positive (Bacillus cereus and Lactobacillus plantarum) bacteria and three fungi (Fusarium oxysporum, Macrophomina phaseolina and Candida albicans). Yellow mustard exhibited higher antibacterial activity while brown mustard had higher antifungal activity. Both the mustard seed meal extracts were ineffective against probiotic bacteria Lactobacillus plantarum. Gallic acid, caffeic acid, ferulic acid and sinapic acid were identified in mustard seed meal by HPLC analysis.