IDENTIFICATION OF POTENTIAL FLUORESCENT PSEUDOMONAS ISOLATES INDUCING DROUGHT TOLERANCE IN RICE AND WHEAT
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Date
2018
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Indira Gandhi Krishi Vishwavidyalaya, Raipur
Abstract
The present investigation was carried out in Microbial Biotechnology Laboratory at the Department of Plant Molecular Biology Laboratory, College of Agriculture, Indira Gandhi Agricultural University, Raipur, Chhattisgarh. Roots are the important part of a plant which were unnoticed in the circle of crop production of cereals. Roots which grow into the complex soil system have to face a lot of obstacles, both abiotic and biotic.The role of microbes in management of biotic and abiotic stresses is gaining importance and they are one of the major parts of soil which shows tremendous different activities in relation with crops. Beneficial microorganisms colonize the rhizosphere / endo-rhizosphere of plants and promote growth of the plants through various direct and indirect mechanisms. The development of stress tolerant crop varieties through genetic engineering and plant breeding is essential but a long drawn process, whereas microbial inoculation to alleviate stresses in plants could be a more cost effective environmental friendly option which could be available in a shorter time frame. Finding the effective isolate which can induce water stress tolerance in host plants requires both laboratory and on field studies. In the present study 31 fluorescent pseudomonas isolates(P1, P2, P3, P4, P5, P6, P7, P8, P10, P11, P12, P13, P14, P16, P17, P18, P19, P21, P22, P23, P24, P25, P27, P28, P29, P30, P66, P141, P200, P229, P260) were tested under different aspects like responds to different biochemical tests, PCR based molecular analysis and actual field performance. PCR based DNA finger printing revealed polymorphism between 31 isolates of fluorescent pseudomonas. All cultures responds variably towards biochemical tests and P5, P7, P8 and P10 were identified as the most potential isolate which can induce water stress tolerance to rice and wheat. These potential isolates can be further used for gene expression studies in host crops and can explore different array of changes.
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