MOLECULAR GENETIC CHARACTERIZATION OF BROILER STRAINS
| dc.contributor.advisor | RAMESH GUPTA, B(MAJOR) | |
| dc.contributor.advisor | SHARMA, R.P | |
| dc.contributor.advisor | HAZARY, R.C | |
| dc.contributor.advisor | NARASIMHA RAO, G | |
| dc.contributor.author | KRISHNA LATHA, K.V. | |
| dc.date.accessioned | 2018-08-23T10:25:40Z | |
| dc.date.available | 2018-08-23T10:25:40Z | |
| dc.date.issued | 2003-09 | |
| dc.description.abstract | ABSTRACT : The potential use of random amplified polymorphic DNA (RAPD) was evaluated as a source of genetic markers for characterization of five genetically distinct broiler strains of chicken to establish genetic relationship among these lines based on the genomic variation observed and to identify strain specific markers, if any. A total of 40 decamer primers were used to amplify the DNA fingerprints in 48 genomic DNA samples of five broiler lines – Cornish High (10), Cornish Mid (10), Cornish Low (5), PB2 (12), a synthetic broiler parent strain and Px (11), a heavy commercial breeder line, maintained at the Project Directorate on Poultry (PDP), Rajendranagar, Hyderabad. The Cornish breed was classified as Cornish High (6.57 0.23), Cornish Mid (5.77 0.11) and Cornish Low (3.63 0.37) based on their antibody titers against Sheep Red Blood Cells (SRBC). Out of 40 oligonucleotide primers screened, 11 primers generated appreciable polymorphism, which were subjected to further analysis. Among a total of 196 bands amplified, 118 (60.2 %) were found to be polymorphic and the number of bands generated per primer varied from 14 to 22. The polymorphism generated by the primers ranged from 35.7 to 81.8 per cent and the size of the amplicons varied from 100 bp to 2600 bp. Analysis of the pooled DNA samples revealed that some of the polymorphic fingerprint profiles generated by the individual DNA samples were found to be absent in the fingerprint profiles generated by the same primer when pooled samples were used, indicating the genetic information of the population rather than the individuals. The analysis of fingerprints generated by the individual genomic DNA samples revealed that the Cornish High line could be distinguished from the others by the presence of a unique 568 bp fragment upon amplification using primer PDP 50F. Cornish Low group was characterized by the absence of 400 bp and 600 bp fragments when amplified by the primers PDP 50F and PDP 73F, respectively. A unique 400 bp fragment was found to be specific to PB2 line when amplified by primer PDP 64F, while the Px line could be characterized by the absence of 100 bp and 580 bp fragments amplified by the primers PDP 73F and PDP 50F, respectively. In addition, the 600 bp and 750 bp fragments were absent specifically in Px line when amplified by the primer PDP 58F. The overall mean genetic similarity within the strains estimated using the individual DNA samples based on Nei’s coefficient method ranged from 81.0 per cent in Px line to 85.53 per cent in Cornish Low and the genetic similarities within the Cornish High, Cornish Mid, Cornish Low, PB2 and Px lines ranged from 59.58 to 82.62, 72.17 to 94.01, 70.01 to 91.50, 64.80 to 92.03 and 63.51 to 86.12 per cent, respectively. The overall mean genetic similarity between the lines using the pooled DNA samples ranged from 77.86 per cent between PB2 and Cornish Mid to 89.54 per cent between Cornish Mid and Cornish Low. The overall mean genetic similarities based on the band frequency (WFi) method in Cornish High, Cornish Mid, Cornish Low, PB2 and Px lines were 61.11, 67.09, 69.44, 64.02 and 67.38 per cent, respectively. The genetic identity indices (BFij) between the five lines varied from 0.63 0.03 between Cornish Low and Px to 0.77 0.04 between Cornish Mid and Cornish Low. The genetic distance between Cornish Low and Px was maximum (0.48 0.05), while the genetic distance between Cornish Low and Cornish Mid was minimum (0.27 0.04). Results of the present study revealed the existence of low genetic variation within the lines and high genetic similarity between the five broiler lines studied. From the present study, it may be concluded that RAPD markers are effective in detecting polymorphisms between and within broiler populations, which is sufficient enough to throw light on their genetic constitution (through sampling) and population structure. This technology provides enough scope for making intermittent genetic assessment of the closed broiler populations of chicken vis-a-vis their selection and breeding histories for facilitating the development of line or breed or trait specific markers for further exploitation. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/5810067125 | |
| dc.keywords | BROILER STRAINS;MOLECULAR GENETIC;CHARACTERIZATION | en_US |
| dc.language.iso | en | en_US |
| dc.pages | 79 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Animal Genetics and Breeding | en_US |
| dc.subject | null | en_US |
| dc.these.type | M.V.Sc. | |
| dc.title | MOLECULAR GENETIC CHARACTERIZATION OF BROILER STRAINS | en_US |
| dc.type | Thesis | en_US |