Screening and characterization of Mesorhizobium ciceri isolates for antagonistic effect to Fusarium oxysporum f. sp. ciceri
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Date
2010
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CCSHAU
Abstract
Screening and characterization of Mesorhizobium ciceri isolates for antagonistic
effect to Fusarium oxysporum f. sp. ciceriā was investigated on 207 isolates obtained from
the nodules of chickpea plant. The growth of these 207 isolates was checked on YEMA
Congo red medium. Out of 207 isolates, 117 were fast growing and 90 were slow growing
rhizobia. These 207 isolates were screened for their anagonistic effect against Fusarium
oxysporum. Out of 207 isolates, seven isolates were found to be antagonistic to F.
oxysporum. Size of inhibition zone varied among the seven isolates from 5 mm to 15 mm.
MC99 produced the largest inhibition zone. These seven isolates were further studied for
antibiotic, siderophore, indole acetic acid (IAA) production and phosphate solubilization.
None of the seven isolates was producing antibiotic. Out of these seven isolates, MC84,
MC96, MC99 were producing siderophore and MC69, MC180, MC183 and MC190 were not
producing siderophore. So, the mechanism of antagonism might be different in all these
isolates. All the seven isolates were produing IAA, which varied among these isolates. The
isolate MC99 was producing maximum indole acetic acid. None of the seven isolates was
solubilizing tricalcium phosphate. In the present study, the isolate MC99 was found to be
having maximum antagonistic activity against F. oxysporum, as it produced maximum zone
of inhibition against this fungus. It had also the maximum amount of IAA and siderophore.