In Vitro Screening for Salt Tolerance in Cotton
| dc.contributor.advisor | H.M. Vamadevaiah | |
| dc.contributor.author | Nirmala | |
| dc.date.accessioned | 2016-11-10T15:35:36Z | |
| dc.date.available | 2016-11-10T15:35:36Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | A laboratory experiment was conducted during 2009-10 in the ‘Tissue culture laboratory’ of Agriculture Research Station, Dharwad Farm, University of Agricultural Sciences, Dharwad to study the effect of in vitro induction of salt stress on growth parameters, osmoprotectant and two enzymes belonging to the class of oxido-reductases in three cotton genotypes viz. Bikeneri Nerma, female parent of NHH44, a popular cotton hybrid for rain-fed situation, AC-738, male parent of same hybrid and Jayadhar, a diploid cotton cultivated under rain-fed conditions. The salinity stress was induced by incorporating different concentrations of salts to generate EC 2, 4, 6 and 12 referred to as T1 to T5, respectively. The experiment was laid out in completely randomised design with three replications. Reduction in seedlings fresh weight, shoot length, root weight, root length and root dry weight was significantly less in Bikaneri Nerma under salt stress as compared to Jayadhar and AC- 738. Salinity stress led to significant increase in proline content in shoots and roots of Bikaneri Nerma when compared to Jayadhar and AC-738. Bikaneri Nerma showed significantly increased Nitrate reductase (NRA) and Peroxidase (POD) in shoots from treatments T1 to T5, whereas in roots the activities of NRA and POD increased up to T2 and T3 onwards the activities declined. The protein expression was studied by SDA-PAGE in all the three genotypes under control and at EC level of 6. The Bikaneri Nerma and Jaydhar genotypes expressed extra protein. Polymorphic difference was also observed among the genotypes. The genotypes studied differed widely in their response to salinity exhibiting thereby that different genotypes may have different adaptation levels against salinity stress.The information generated from the present investigation reveals that the genotypes Bikaneri Nerma and Jayadhar may be better suited for salinity stress conditions. | en_US |
| dc.identifier.uri | http://krishikosh.egranth.ac.in/handle/1/84928 | |
| dc.publisher | UAS, Dharwad | en_US |
| dc.research.problem | In Vitro Screening for Salt Tolerance in Cotton | en_US |
| dc.sub | Plant Biochemistry | en_US |
| dc.subject | Plant Biochemistry | en_US |
| dc.theme | In Vitro Screening for Salt Tolerance in Cotton | en_US |
| dc.title | In Vitro Screening for Salt Tolerance in Cotton | en_US |
| dc.type | Thesis | en_US |