Phenotypic and molecular analysis for terminal heat stress tolerance in wheat (Triticum aestivum L.)
Loading...
Date
2013
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CCSHAU
Abstract
The present investigation was conducted to study the phenotypic and molecular analysis of terminal heat stress tolerance in wheat (Triticum aestivum L.) in the Department of Molecular Biology & Biotechnology, CCS Haryana Agricultural University, Hisar, India. Two F2 mapping populations containing 90 plants each involving crosses between Hindi62 (heat tolerant) x WH711 (heat susceptible) and DBW16 (heat tolerant) x WH147 (heat susceptible) were developed. Seeds from individual F2 plants were harvested and sown in two environments, normal sown (30th November 2011) and late sown (26th December 2011) to get F3 mapping population. Heat stress was created by late sowing the crop. Data was recorded on individual plant basis for both the crosses in respect of characters viz. membrane thermostability index, chlorophyll stability index, grain yield, kernels/spike, thousand kernel weight (Test weight), days to heading, days to maturity, plant height, number of tillers/plant, biomass/plant, harvest index, grain filling rate and grain filling duration.103 SSR, 25 RAPD and 2 known gene primers were used in molecular marker analysis for terminal heat stress tolerance. For QTL identification data was analysed by WinQTL-Cartographer. In cross Hindi62 x WH711 three QTL were detected for chlorophyll index at map positions, 135.7 (1B), 184.6 (2A) and 36.4 (6B) by SSR marker WMC 416, Xbarc188, Xgwm526, WMC170, Xgwm219, WMC398respectively. Six QTL were detected for membrane thermostability index at map positions, 118.7 (1B), 58.2 (2A), 137.2 (2A), 176.7 (2A), 62.0 (5A) and 11.7 (6B) by SSR marker WMC416, Xbarc188, WMC407, WMC642, Xgwm356, Xgwm95, Xgwm526, WMC170, Xgwm443, WMC475, Xgwm219 and WMC398. For yield per plant 6 QTLs were detected at map position103.4 (1B), 51.6 (2A), 130.6 (2A), 178.9 (2A), 55.4 (5A), 22.6 (6B). For grain filling duration one QTL was detected at chromosome no. 2A at a map position 171.9 and for grain filling rate 2 QTLs were detected on chromosome 2A and 6B at map position of 66.0 and 76.3 respectively. Similarly in cross DBW16 x WH147, 4 QTLs were detected for chlorophyll index on chromosome 1B, 2A, 6B and 6B respectively. Five QTLs were for membrane thermostability index on chromosome 1A, 1B, 2A, 3A and 6B.One QTL was identified for yield per plant and one for grain filling rate at chromosome 2A and 4A respectively. Five QTLs were identified for grain filling duration at a map position 113.5 (2A), 31.5 (2A), 88.5 (3A), 31.0 (5A) and 29.0(5B). These QTLs may be used for further improvement of the traits they represent. HSP16.9 and HSP 101b primers showed clear distinction between thermotolerant and thermosensitive genotypes and are suggested as potential DNA markers for improving thermotolerance in wheat using marker-assisted selection.
Description
Keywords
Planting, Developmental stages, Wheats, Grain, Genetics, Biological phenomena, Tolerance, Packaging, Yields, DNA