Phytochemical Analysis and Possible Synergistic Interactions of Vitex agnus-castus L., Vitex negundo L., and Piper longum L. Essential oils on Pesticidal Activities

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dc.contributor.advisorKumar, Ravinder
dc.contributor.authorBargali, Pooja
dc.date.accessioned2024-08-23T10:25:43Z
dc.date.available2024-08-23T10:25:43Z
dc.date.issued2023-07
dc.description.abstractThis study focused on the phytochemical analysis and possible synergistic interactions of EOs from Piper longum L., Vitex negundo L., and Vitex agnus-castus L. on pesticidal activities. The plant material was collected from the Tarai region of Uttarakhand. The EOs were extracted through hydro-distillation using a Clevenger type of apparatus. The EOs yields obtained from 0.04% to 0.42%. The phytochemical analysis of the EOs was done by GC and GC-MS. The GC-MS analysis of PLAO, VNAO, and VACAO identified 37, 97, and 47 compounds constituting 98.8%, 98.8%, and 99.8% of the total EOs. The major compounds identified in PLAO were β-pinene, cis-nerolidol, 2-heptyl acetate, α-pinene, (E)-caryophyllene, limonene, 2-undecanone, b-eudesmol, linalool, camphene, etc. Viridiflorol, β-caryophyllene, labd-8-en-15-oic acid, ethyl ester, 5-(1-isopropenyl-4,5- dimethyl bicyclo[4.3.0]nonan-5-yl)-3-methyl-2-pentenol acetate, caryophyllene oxide, 2- trans-octenol, trans-β-ocimene, 4-terpineol, and phytol, etc. were identified as major compounds in VNAO. Similarly, 1,8-cineole, (E)-β-ocimene, α-terpinyl acetate, terpinen-4- ol, (Z)-β-ocimene, α-terpineol, β-caryophyllene, trans-β-farnesene, g-terpinene, etc. were identified as major compounds in VACAO. The recovered EOs were blended in binary and ternary mixtures at equal ratios to analyze their synergistic interactions on pesticidal activities. The nematicidal and antifungal activities of the EOs were evaluated using standard protocols. The nematicidal activity of the individual EOs and their combinations was assessed against root-knot nematodes M. incognita at different concentrations (0.25, 0.5, and 1 μL/mL). The antifungal activity of pure and blended EOs was also tested against two phytopathogenic fungi, Rhizoctonia solani, and Sclerotinia rolfsii, using the discdiffusion technique at different concentrations (40, 60, and 80 μL/mL). Furthermore, a molecular docking study was conducted on the major compounds of the EOs to investigate their interactions with target proteins in comparison with standard inhibitors physostigmine (AChE), ethacrynic acid (GST-1), and 14 α-demethylase enzyme (CYP51). Overall, this study provides valuable information into the nematicidal and antifungal potential of PLAO, VNAO, VACAO, and their binary or ternary combinations. The results of our investigation confirm synergistic interactions among the tested EOs. These findings will be implications for sustainable agriculture and the development of natural products with pesticidal properties.
dc.identifier.citationTheses of M.Sc.
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810213659
dc.keywordsPesticide
dc.language.isoEnglish
dc.pages187 p.p.
dc.publisherG. B. Pant University of Agriculture & Technology, Pantnagar-263145
dc.relation.ispartofseries10880
dc.subChemistry
dc.themeAcademic Research
dc.these.typeM.Sc
dc.titlePhytochemical Analysis and Possible Synergistic Interactions of Vitex agnus-castus L., Vitex negundo L., and Piper longum L. Essential oils on Pesticidal Activities
dc.typeThesis
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