Identification, characterization and expression analysis of calmodulin and calmodulin-like protein genes in chickpea (Cicer arietinum L.)

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dc.contributor.advisorSharma, Kamal Dev
dc.contributor.authorVerma, Avnika
dc.date.accessioned2023-02-01T05:40:31Z
dc.date.available2023-02-01T05:40:31Z
dc.date.issued2022-12-16
dc.description.abstractChickpea (Cicer arietinum L.), is an important legume crop grown widely for its nutritious seeds. It is believed to be originated in South-West Asia. Chickpea productivity is severely hampered by both abiotic (cold, heat, draught, salt) and biotic stresses (wilt & blight). Following stress, the plant cells exhibit Ca2+ fluxes. The Ca2+ binds to the calcium binding proteins such as Calmodulin and Calmodulin-like leading to Ca2+ induced signaling and plant’s responses to stress. In the present investigation, Ca2+ binding genes, Calmodulin (CaM) and Calmodulin-like (CML) in chickpea were identified and characterized. Four calmodulin and thirty-eight calmodulin-like genes were identified in the chickpea genome. The Calmodulin and Calmodulin-like genes were distributed in all the eight chromosomes of chickpea while chromosomal location of four Calmodulin-like genes could not be ascertained. A new nomenclature for these genes in chickpea was also proposed because the previous nomenclature was erroneous and confusing. The gene, mRNA, protein and coding sequences were identified for each genes using appropriate database and software, and the gene identities were confirmed using NCBI, SMART and Pfam tools. Gene structure, phylogenetic relationships and protein-protein interaction were also deduced by using appropriate bioinformatics tools. Expression of four CaM (Calmodulin) and thirty-eight CML (Calmodulin-like) genes was studied in five different organs of chickpea (leaf, stem, root, anther and ovule). CaM and CML gene families in chickpea expressed differentially in different organs. Expression analysis showed that maximum number of CaM and CML genes expressed at very high levels in roots and ovules showing root and ovule specificity. One CaCaM and 14 CaCMLs genes overexpressed in ovule, 2 CaCaM and 13 CaCMLs genes overexpressed in root, 4 CaCMLs genes overexpressed in leaf, 3 CaCMLs genes overexpressed in anther and 2 CaCMLs genes overexpressed in anther. The study revealed the existence of large number of CaCaM and CaCML genes in chickpea and that these genes express in organ specific manner suggesting organ specific function of each gene.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810192999
dc.keywordsChickpea, Cicer arietinum L., Calmodulin, Protein genesen_US
dc.language.isoEnglishen_US
dc.pages97en_US
dc.publisherCSK HPKV, Palampuren_US
dc.subAgricultural Biotechnologyen_US
dc.themeThe objectives of the present study are: Genome wide identification and characterization of Calmodulin and Calmodulin-like protein gene in chickpea; & Elucidation of expression of calmodulin and calmodulin-like protein genes in chickpea.en_US
dc.these.typeM.Scen_US
dc.titleIdentification, characterization and expression analysis of calmodulin and calmodulin-like protein genes in chickpea (Cicer arietinum L.)en_US
dc.typeThesisen_US
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