STUDIES ON CULTIVATION OF SHIITAKE [Lentinula edodes (Berk.) Singer] MUSHROOM
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Date
2019-10
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UHF,NAUNI
Abstract
ABSTRACT
The test fungus Lentinula edodes (OE-388), was evaluated with respect to their cultural
requirements and the cultivation aspect. Cultural studies of test fungi were carried out under in vitro
conditions. Our findings revealed that average mycelial weight (260 mg/100 ml) of media was
recorded highest at pH 6, under in vitro conditions.. The temperature of 25 °C supported maximum
mycelial growth whereas 30°C and higher was inhibitory to the growth of L. edodes. In solid media
studies maximum radial growth (8.82 cm) was obtained with potato dextrose agar while minimum
growth was recorded in Czapek’s Dox agar. Out of different substrates tested for spawn production,
wheat grain took minimum number of days for completion of spawn run followed by bajra while saw
dust took maximum number of days for spawn development. Among the different substrates tried, the
maximum mycelium running rate of 4.08 mm/day was in oak saw dust and minimum of 2.18 mm/day
was in wheat straw. Willow saw dust produced the maximum 12 number of average fruit bodies per
bag followed by poplar having 11.25. Sugarcane bagasse has lowest number (1.97) of fruiting bodies
per bag. Poplar produced maximum of 18.34 g average fruit body weight followed by the willow
14.88g. Minimum average weight of 7.74g was found in the sugarcane bagasse. Maximum Average
pileus thickness and diameter was found in willow saw dust and Sugarcane bagasse has maximum
stalk length of mushroom ranges from 5.87-6.20. Maximum stalk diameter of 1.19cm was observed in
poplar saw dust substrate and stalk diameter ranges between 1.02-1.20 cm. The highest average yield
was recorded in willow saw dust (194.16 g) followed by poplar and lowest average yield (30.83) was
found in sugarcane bagasse. Among the different supplementations used saw dust with wheat bran
and CaCO3 showed highest biological efficiency followed by corn cobs.
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