STUDIES ON CULTIVATION OF SHIITAKE [Lentinula edodes (Berk.) Singer] MUSHROOM

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Date
2019-10
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UHF,NAUNI
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ABSTRACT The test fungus Lentinula edodes (OE-388), was evaluated with respect to their cultural requirements and the cultivation aspect. Cultural studies of test fungi were carried out under in vitro conditions. Our findings revealed that average mycelial weight (260 mg/100 ml) of media was recorded highest at pH 6, under in vitro conditions.. The temperature of 25 °C supported maximum mycelial growth whereas 30°C and higher was inhibitory to the growth of L. edodes. In solid media studies maximum radial growth (8.82 cm) was obtained with potato dextrose agar while minimum growth was recorded in Czapek’s Dox agar. Out of different substrates tested for spawn production, wheat grain took minimum number of days for completion of spawn run followed by bajra while saw dust took maximum number of days for spawn development. Among the different substrates tried, the maximum mycelium running rate of 4.08 mm/day was in oak saw dust and minimum of 2.18 mm/day was in wheat straw. Willow saw dust produced the maximum 12 number of average fruit bodies per bag followed by poplar having 11.25. Sugarcane bagasse has lowest number (1.97) of fruiting bodies per bag. Poplar produced maximum of 18.34 g average fruit body weight followed by the willow 14.88g. Minimum average weight of 7.74g was found in the sugarcane bagasse. Maximum Average pileus thickness and diameter was found in willow saw dust and Sugarcane bagasse has maximum stalk length of mushroom ranges from 5.87-6.20. Maximum stalk diameter of 1.19cm was observed in poplar saw dust substrate and stalk diameter ranges between 1.02-1.20 cm. The highest average yield was recorded in willow saw dust (194.16 g) followed by poplar and lowest average yield (30.83) was found in sugarcane bagasse. Among the different supplementations used saw dust with wheat bran and CaCO3 showed highest biological efficiency followed by corn cobs.
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