GENETICS AND MAPPING OF ANTHRACNOSE RESISTANCE GENE(s) IN COMMON BEAN LANDRACE KRC5
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Date
2015-08-14
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CSKHPKV, Palampur
Abstract
ABSTRACT
KRC5, a local landrace of common bean native to Kinnaur region of north western Himalayan state Himachal Pradesh is a well known for its effectiveness against eight pathogen races of C. lindemuthianum viz., 73, 83, 903, 591, 647, 775, 931 and 935. This study reports the identification, mapping and physical delimitation of the chromosomal location of a new anthracnose resistance gene Co-ind from landrace KRC5. The segregation analysis of an F2 progeny of a cross between a susceptible cv. ‘Jawala’ and resistant KRC5, an Andean genotype with three different races viz., 3, 537, 935 suggested that the resistance was conditioned by a single dominant gene. Bulk segregant analysis and recessive class analysis based strategy was used for the mapping of R gene. Preliminary mapping by linkage analysis of 159 F2/ RIL individuals with marker OPF6522 and OPR151136 localized the anthracnose resistance gene on chromosome 7. The R-locus was bracketed between the markers ScOPF6522 and OPR151136 located at a distance of 4.4 and 5 cM, respectively. In this region, six putatively expressed, LRR kinases and serine/threonine kinases (SRK) were short-listed as a potential candidate for the resistance gene identified from KRC5. Out of 178 accession phenotypically evaluated for resistance to race 3 of C. lindemuthianum, only five accessions viz., IC260292, IC265938, IC47839, EC400403 and EC325078 exhibited the presence of Co-ind. In R-gene postulation assay using SCAR markers SCO08, SZ20, SF10 and SAS13, bean accession IC262769 was identified as natural gene pyramid harboring four anthracnose resistance genes (Co-4, Co-42, Co-6 and Co-10) whereas 10 accessions viz., IC260336, IC258273, IC262748, IC47839, EC500328, EC4003433, EC325078 IC265940, IC202271 and IC265938 showed the presence of three resistance genes each. These multigenic accessions represent potential resistance sources and can be effectively utilized either directly as varieties after agronomic evaluation or can be involved in hybridization programmes to transfer resistance in susceptible genotypes after further validation.
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P.126
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