USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

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dc.contributor.authorUma, A
dc.contributor.authorParthiban, M
dc.contributor.authorChandran, Daniel Joy N
dc.contributor.authorThiyagarajan, B
dc.contributor.authorArasi, Kalaimani P
dc.contributor.authorKoteeswaran, A
dc.contributor.authorTANUVAS
dc.date.accessioned2018-12-27T11:43:32Z
dc.date.available2018-12-27T11:43:32Z
dc.date.issued2005-07
dc.descriptionTNV_IJAR_2005_39(2)111-114en_US
dc.description.abstractMonodon baculovirus (MBV) affects the cultured shrimp and results in production and economic losses in the shrimp hatcheries and farms. Microscopic observation of the malachite green stained wet mount squashes from the hepatopancreas for the presence of MBV occlusion bodies is the widely followed method to diagnose MBV. This method was compared with a sensitive nested PCR assay to grade the level of MBV infection in shrimp seeds (postlarvae). Out of 100 shrimp seed samples screened for MBV, 20% of the seed samples were found to be positive for MBV by both microscopic observation and I step PCR whereas 30% of the shrimp seeds samples were found to be positive for MBV by II step or nested PCR only, The op'imum concentration of DNA to be used for nested PCR assay for diagnosis of MBV was also standardized in this study.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810086503
dc.keywordsNested Polymerase, Chain Reaction, Monodon Baculovirus, Shrimp Seedsen_US
dc.language.isoenen_US
dc.pages111-114en_US
dc.publisherAgricultural Research Communication Centreen_US
dc.relation.ispartofseries;2
dc.subjectVeterinary Scienceen_US
dc.titleUSEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDSen_US
dc.title.alternativeIndian Journal of Animal Researchen_US
dc.typeArticleen_US
dc.volume39en_US
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