Isolation and Characterization of Vibrio spp. from Sea Food And Environmental Samples in and around Chennai City
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Date
2021-01
Journal Title
Journal ISSN
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Publisher
TANUVAS, Chennai
Abstract
Vibrio is a major sea food pathogen which needs to be monitored regularly for
devising appropriate control strategy to ensure food safety among sea food consumers and
handlers. The present study aims to ascertain the occurrence of Vibrio spp. in seafood viz
fishes, prawn/shrimps, crabs and water samples collected from various fish markets in and
around Chennai city, Tamil Nadu, India. A total of 175 samples were screened, of which 106
(60.57%) samples were found positive by PCR targeting the 16s rRNA of Vibrio genus and all
the positive isolates were subjected to toxR gene identification by Multiplex PCR for species
level identification. At the species level 28(26.41%) were positive for Vibrio parahemolyticus
(Vp), 6 (5.67%) positive for Vibrio Cholerae (Vc), 2 (1.89%) positive for Vibrio vulnificus
(Vv). The presence of mixed vibrio species were also observed of which 56 samples (43.40%)
revealed the presence of Vp and Vc, 7 (6.60%) with Vp and Vv, 3 (2.84%) with Vv and Vc
and 14 samples with (13.20%) Vp, Vv and Vv. The present study identified the presence along
with mixed Vibrio spp. in most of the samples tested from the study area.
Description
TNV_IJVASR_50(1)_Jan-Feb,2021_38-44
Keywords
Veterinary Science, Veterinary Public Health and Epidemiology