STUDIES ON SEED SET AND COLCHICINE CONTENT IN Gloriosa superba L.
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Date
2021-08
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UHF,NAUNI
Abstract
ABSTRACT
The present research entitled “Studies on seed set and colchicine content in Gloriosa superba L.”
was carried out in the experimental field as well as in laboratory of the Department of Forest Products, College
of Forestry, Dr Y S Parmar University of Horticulture and Forestry, Nauni, Solan (HP) during 2019-21. The
study was conducted on morphology, phenology, floral biology, seed germination, pollination treatment and
colchicine content in different parts of Gloriosa superba. The species was perennial in nature with climbing
stem. Stem was cylindrical, weak, glabrous, sparsely branched arising from V or L shaped tubers. Leaves were
simple, ovate to lanceolate, sessile, entire, glabrous, obtuse base with acuminate tips endings into tendrils.
Flowers were greenish at first then yellow passing through orange to scarlet red colour at maturity. The
maximum stigma receptivity was found on the day of anthesis and maximum anthers dehisced between 8:30-
9:30 am. The peak period of anthesis was found at the time of 8:30 to 9:30 am. In bed conditions, maximum
germination percentage (64.80 %), height of seedling (18.35) cm, number of leaves per seedling (15.70), peak
period of germination during fifth week was found in hot water without sarcotesta (T6) treatment, earlier
germination initiation and minimum germination duration was recorded in conc. H2SO4 (T10). The maximum
micro-tuber length and diameter (4.92 cm), (8.87 mm) of arm one and length and diameter of arm two (3.09
cm), (8.45 mm) was recorded in hot water without sarcotesta treatment (T6). Similarly, in pot condition,
maximum germination percentage (62.40%), earlier germination initiation (32.60 days), height of seedling
(18.05 cm), number of leaves per seedling (16.10), peak period of germination during sixth week was observed
in hot water without sarcotesta treatment (T6) and minimum germination duration (30.30 days) was observed in
PGPR-I (T2). The maximum micro-tuber length and diameter (4.56 cm), (8.35 mm) of arm one and length and
diameter (2.64 cm), (7.89 mm) of arm two was observed in hot water without sarcotesta (T6) treatment. Weight
of micro-tuber was recorded maximum (3.48 g) in bed as well as in pot condition (3.13 g) in treatment of hot
water without sarcotesta (T6). Among different pollination methods, maximum fruit set (80.00 %), number of
seeds per pod (43.73), pod length (6.04 cm), pod diameter (1.81cm), 100 seed weight (3.36 g), seed diameter
(4.29 mm), extract percent (34.44 %), colchicine content (1.54 %) was noticed in assisted pollination treatment
(T7). Assisted pollination method resulted in maximum amount of colchicine content in seeds (1.40 %) which
was followed by pericarp (0.29 %), leaves (0.11 %) and stem (0.03 %). The seeds without sarcotesta also
resulted in high percentage (0.94 %) whereas with sarcotesta value of colchicine recorded was (0.43 %).