Development of an efficient plant regeneration system in kinnow mandarin (Citrus nobilis lour. × citrus deliciosa tenora)
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Date
2012
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Publisher
CCSHAU
Abstract
The present study was undertaken to develop an efficient plant regeneration system in
Kinnow mandarin (Citrus nobilisLour. × Citrus deliciosaTenora). The shoot tip and nodal
explants were cultured on MS medium supplemented with different concentrations and
combinations of growth regulators. Sixteen media combinations were tried for multiple shoot
induction. Highest percent shoot response for nodalexplant (72.25%) was observed on MS
medium supplemented with BAP (2.5 mg/L) and NAA (0.2 mg/L). In case of shoot tip
explant, highest percent shoot response (60.72%) was on MS medium supplemented with
BAP (2.5 mg/L) + NAA (0.2 mg/L). It was observed that the plant regeneration was
influenced by the type of explant and medium. Maximum number of shoots per explant in
case of nodal segments were 5.43 on medium fortified with BAP (1.0 mg/L). While in shoot
tip explants maximum number of shoots per explant were 4.66 on medium supplemented with
Kinetin (2.5 mg/L) and NAA (0.2 mg/L). The nodal explants showed better shoot
regeneration response than shoot tip explants. Twelve rooting media were tried for root
formation in regenerated shoots. Maximum rooting response was 83% on half MS medium
supplemented with IBA (1.0 mg/L) in both the explants. The regenerated plants were
transferred to a mixture of sand: soil in 1:1 ratio in pots and 73.33% regenerated plants
survived in the soil.
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Keywords
Fruits, Regeneration, Auxins, Planting, Biological phenomena, Vegetative propagation, Cytokinins, Biological development, Concentrates, Developmental stages