Synthesis, characterisation and pharmacological assessment of nanoparticles of Tinospora cordifolia for its cytotoxic activity
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Date
2020
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Abstract
The present study was taken up to evaluate the use of Tinospora cordifolia stem extract as a reducing
agent for silver nanoparticle formation and characterization of the synthesized nanoparticles and
assessment of its cytotoxic effect on the HepG2 cancer cell line. Silver nanoparticles were synthesized
and characterized by visualizing the colour change, observing for the concentric rings under an optical
microscope, analyzing the particle size and measuring the zeta potential. The extract was also
phytochemically characterized and subjected to MTT assay to evaluate its cytotoxic activity at three
different doses of 200 μg/ml, 400 μg/ml and 600 μg/ml against a negative control and a positive control
5-Flurouracil 5 μg/ml. The synthesized nanoparticles were confirmed by visualizing the colour change
from colourless to slight reddish brown colour, observing concentric rings under optical microscope,
determining the particle size in the range of 0.4 nm and zeta potential was measured to be -13.4mv with
peak area of 100 percentage intensity. Phytochemical analysis revealed the presence of saponin,
terpenoids, flavanoids, hydrolysable tannin, glycosides and cardiac glycosides in the aqueous extract. The
cytotoxicity assay revealed a dose dependent significant decrease in the percentage inhibition of growth
of cells with the highest level of inhibition noticed at the highest dose similar to positive control group.
Thus the cytotoxic effect of the extract could be attributed to the presence of the secondary metabolites in
them which could get converted into nanoparticles with silver and establishes its potential as a
chemotherapeutic option for cancer.
Description
TNV_JPP_2020_9(3)1901-1906
Keywords
Veterinary Science