SCREENING OF SWEET SORGHUM (Sorghum bicolor L.) GENOTYPES FOR HIGHER ETHANOL PRODUCTION
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Date
2015-07-30
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UNIVERSITY OF AGRICULTURAL SCIENCES GKVK, BENGALURU
Abstract
Due to the increasing price and depletion of fossil fuels there a necessity to find
an alternative source of fuel. Sweet sorghum (Sorghum bicolor (L.) Moench) which is
similar to grain sorghum with sugar-rich stalks and a water-use efficient crop has a very
good potential as an alternative feed stock for ethanol. Production cost of cultivation of
sweet sorghum is 3 times lower than sugarcane. Similar to sugarcane, sweet sorghum
juice extracted from stalks is high in sugars that are readily fermentable. In the present
study fifteen sweet sorghum genotypes were screened for higher ethanol production.
Genomic DNA was isolated using CTAB (2 %) method from young leaves. These
genotypes were analysed for genetic diversity using SSR markers. SSR markers have
particular importance because of their abundant distribution, highly polymorphic,
reliable, and co-dominance nature. Matured plants juice was extracted from the stalk of
the plants, RSSV 138 and URJA genotypes showed highest total sugar concentration of
14.78 % and 14.39 % respectively. The juice was fermented using Saccharomyces
cerevisiae among which RSSV 138 and CSH 22SS genotypes showed highest ethanol
yield of 13.25 g/l and 13.01 g/l respectively. From this study the sweet sorghum
genotypes having high sugar concentration were identified and can be used for bioethanol
production.
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