ISOLATION AND CHARACTERIZATION OF VIBRIO SPECIES FROM FISH AND FISH POND WATER
| dc.contributor.advisor | Bindu Kiranmayi, CH (MAJOR) | |
| dc.contributor.advisor | Srinivasa Rao, T | |
| dc.contributor.advisor | Aswani kumar, K | |
| dc.contributor.author | SUSMITHA, BUJUTI | |
| dc.date.accessioned | 2022-09-26T11:31:10Z | |
| dc.date.available | 2022-09-26T11:31:10Z | |
| dc.date.issued | 2022-03 | |
| dc.description | THESES | en_US |
| dc.description.abstract | The consumption of raw, improperly cooked or cross-contaminated fish and fish products by Vibrio spp. can cause foodborne diseases. The present study was undertaken to characterize Vibrio species of fish and fish pond water origin based on cultural isolation. It was also aimed to determine the major antibiotic resistant genotypes and virulence genes of Vibrio species of worldwide public health importance. A total of 300 samples comprising fish and fish pond water samples were analyzed for presence of Vibrio spp. viz., V. parahaemolyticus, V. alginolyticus, V. cholerae and V. vulnificus. Overall prevalence of genus Vibrio was found to be 55% (165/300). Out of 165 Vibrio isolates, m-PCR revealed 28 (16.9%) to be V. parahaemolyticus, 17(10.3%) to be V. alginolyticus and 15 (9.09%) to be V. cholerae and 11 (6.66%) to be V. vulnficus. Out of 131 positive Vibrio spp. in fish, highest prevalance was found in Catla catla (54.28%) followed by Labeo rohita (53.75%), Channa striata (48.38%) and other fishes (37.20%). Virulence genes are major indicators to pathogenicity of these microorganisms. Virulence genes ctxAB, tcp, rfbO1, zot were not detected in any of the V. cholerae isolates. Out of 28 V. parahaemolyticus isolates, virulence genes trh and tdh were detected in 21.42% and 10.71% of isolates, respectively. Out of 17 V. alginolyticus isolates, one (5.88%) isolate was carrying tdh gene. Out of 11 V. vulnificus isolates, cps allele 2 and vcgE were detected in 27.27% and 18.18% respectively and 18.18% of isolates were positive for both cps allelle 2 and vcg E. Antibiogram profiling of 71 isolates revealed a major fraction of the Vibrio isolates to be resistant towards ampicillin (100%) followed by amoxicillin/clavulanic acid (91.54%) and erythromycin (52.11%). ESBL production was confirmed in 15 Vibrio spp. isolates by both phenotypic and molecular methods and blaTEM was the predominant gene in 14 isolates and blaOXA gene was detected in only one isolate. A greater degree of heterogeneity was observed among 71 Vibrio isolates of different species from different sources as revealed by presence of 70 genotypes and 71 genotypes by ERIC and REP-PCR analysis, respectively. Twenty seven different V. parahaemolyticus, 17 V. alginolyticus, 15 V. cholerae and 11 V. vulnificus subtypes were differentiated by ERIC-PCR, whereas 28 different V. parahaemolyticus, 17 V. alginolyticus, 15 V. cholerae and 11 V. vulnificus subtypes by REP- PCR. Genotyping of Vibrio species by ERIC- PCR and REP- PCR was found to be highly significant since discriminatory power >0.9 is considered highly significant (0.9974 for ERIC PCR and 1 for REP-PCR. Cluster analysis also revealed a great degree of homogeneity and heterogeneity among different isolates recovered from different sources and indicating that there is a chance of cross-contamination particularly in the fish markets | en_US |
| dc.identifier.uri | https://krishikosh.egranth.ac.in/handle/1/5810188183 | |
| dc.keywords | ISOLATION; CHARACTERIZATION; VIBRIO SPECIES; FISH; POND WATER | en_US |
| dc.language.iso | English | en_US |
| dc.pages | 179 | en_US |
| dc.publisher | SRI VENKATESWARA VETERINARY UNIVERSITY TIRUPATI - 517 502. (A.P.) INDIA | en_US |
| dc.sub | Veterinary Public Health | en_US |
| dc.theme | ISOLATION AND CHARACTERIZATION OF VIBRIO SPECIES FROM FISH AND FISH POND WATER | en_US |
| dc.these.type | M.V.Sc. | en_US |
| dc.title | ISOLATION AND CHARACTERIZATION OF VIBRIO SPECIES FROM FISH AND FISH POND WATER | en_US |
| dc.type | Thesis | en_US |