LARGE SCALE PRODUCTION OF Cry 1Aa PLANTS IN SORGHUM

Simple item page
dc.contributor.advisorANURADHA, G
dc.contributor.authorKAVITHA, A
dc.date.accessioned2016-08-24T10:01:31Z
dc.date.available2016-08-24T10:01:31Z
dc.date.issued2005
dc.description.abstractSorghum is an important dryland crop used as food, feed and fodder. The increasing shift in the demand for sorghum from staple food to industrially preferred commodity in poultry, bakery and brewing industries requires large scale production according to the needs of different sectors. Therefore, it is important to develop and deploy novel technologies such as genetic transformation to increase the grain quality and resistance to pests that are difficult to breed by conventional means. Two principal methods viz., Agrobacterium infection and particle bombardment were used for genetic transformation. In vitro response of sorghum was low, monocalcitrant and was highly genotype dependent. Tissue culture and regeneration response was low in sorghum when compared to other cereals. Agrobacterium method of transformation was preferred because of its cost efficacy and low copy number. But regeneration of explants was very low due to overgrowth of Agrobacterium. Hence during the course of study alternate methods of Agrobacterium infection were studied using two inbred lines AKMS 14B and 296B. Different media adjuvants were studied to control Agrobacterium growth. AgNO3 and CuSO4 were effective in controlling the bacterial growth. Gus expression was studied in cotransformation experiments using two individual genes viz., pDM805 and pCAMBIA 3300 cry 1Aa. Gus results revealed that they were independently transformed without influencing each other. A non tissue culture approach was done to produce transformants at large scale. Though transient gus expression was present in the immature embryos, mature seeds were not selected in basta screening. Putative transformants were evaluated through basta spray tests and PCR analysis using bar and Cry 1B primers. Results of basta swab test were in agreement with PCR results.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/73460
dc.language.isoenen_US
dc.publisherACHARYA N. G. RANGA AGRICULTURAL UNIVERSITY, RAJENDRANAGAR, HYDERABADen_US
dc.relation.ispartofseriesD7427;
dc.subAgricultural Biotechnology
dc.subjectLARGE, SCALE, PRODUCTION, Cry, 1Aa, PLANTS, SORGHUMen_US
dc.these.typeM.Sc
dc.titleLARGE SCALE PRODUCTION OF Cry 1Aa PLANTS IN SORGHUMen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
D7427.pdf
Size:
1.88 MB
Format:
Adobe Portable Document Format
Description:
LARGE SCALE PRODUCTION OF Cry 1Aa PLANTS IN SORGHUM
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Theses