MORPHOGENETIC VARIATION OF SEABUCKTHORN (Hippophae rhamnoides L.) IN SPITI VALLEY OF HIMACHAL PRADESH

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dc.contributor.advisorSANKHYAN, H.P.
dc.contributor.authorKAIRON, VINOD KUMAR
dc.date.accessioned2018-01-19T06:46:18Z
dc.date.available2018-01-19T06:46:18Z
dc.date.issued2017-12
dc.description.abstractABSTRACT The study entitled “Morphogenetic Variation of Seabuckthorn (Hippophae rhamnoides L.) in Spiti Valley of Himachal Pradesh” was conducted during the year 2014-17 with the aim to evaluate morphological variation, variation in fodder quality traits, variation in physical and chemical characteristics of berries, seed oil and deoiled cake and and molecular characterization of morphovariants using RAPD of different gene pools of Hippophae rhamnoides under different gene pool areas and growing conditions. Nine gene pool areas and three growing conditions within gene pool areas selected for the study showed wide variation among different morphological, fodder quality traits, physical and chemical characteristics of berries, seed oil and deoiled cake. Morphological traits (plant height, leaf length, leaf width, leaf area, leaf density, no. of thorn, 100 fruits fresh weight, fruit diameter and 100 seed weight) differed positively significant among the gene pool areas and growing conditions, except branch length. All proximate leaf composition (100 leaf fresh weight, 100 leaf dry weight, crude fiber, crude protein and total ash) and mineral nutrient (nitrogen, potassium, calcium and magnesium) compositions showed significant difference between the gene pool areas and growing conditions for all the parameters except for leaf dry matter content and phosphorus. All physical and chemical characteristics of berries (pulp%, ascorbic acid and total sugar content), per cent seed oil, physico-chemical traits of oil (specific gravity, acid value, iodine value and saponification value) and deoiled seed cake (protein content, starch content and total sugar content) showed significant under different gene pool areas and growing conditions for all the traits, except for physical characteristics (refractive index and specific gravity) of seed oil. The RAPD fragments obtained after the amplification of genomic DNA for 27 genotypes. Total 89 RAPD markers were amplified and 71 out of 89 were found to be polymorphic in nature. The size range of polymorphic bands was 100 bp to 2,500 bp. The Jaccard's similarity correlation coefficient values ranged from 0.520 to 0.879. The highest value (0.879) was found between 87.9 per cent was found between GPA3GC1 and GPA3GC2 followed by 0.853 between GPA4GC1 and GPA8GC1 genotypes. In overall, GPA4 (Schilling) gene pool area proved to be the best among different gene pool areas on the basis of fodder quality traits. GPA3 (Sheigo) was found to be best in all gene pool areas for physical and chemical characteristics of berries, seed oil and deoiled seed cake. Among different growing conditions, GC3 (Crop Land) growing condition was found to be top ranking for most of morphological traits, fodder quality traits, and physical and chemical characteristics of berries, seed oil and deoiled seed cake. So Seabuckthorn can be used as agroforestry crop in different systems and designs. Based on the morphological variation, variation in fodder quality traits, variation in physical and chemical characteristics of berries, seed oil and deoiled cake observations it was evident that large diversity exists in naturally growing populations of Hippophae rhamnoides in the Spiti Valley of Himachal Pradesh, and this morphological variation can be exploited for the further selection of suitable genotypes to accelerate the domestication process of this important species.en_US
dc.identifier.urihttp://krishikosh.egranth.ac.in/handle/1/5810039652
dc.keywordsSeabuckthorn (Hippophae rhamnoides L.),Morphogenetic Variation,Spiti Valley of Himachal Pradeshen_US
dc.language.isoenen_US
dc.pages110+VIIen_US
dc.publisherUHF,NAUNIen_US
dc.subTree Improvement and Genetic Resourceen_US
dc.subjectnullen_US
dc.themeSeabuckthorn (Hippophae rhamnoides L.),Morphogenetic Variation,Spiti Valley of Himachal Pradeshen_US
dc.these.typePh.Den_US
dc.titleMORPHOGENETIC VARIATION OF SEABUCKTHORN (Hippophae rhamnoides L.) IN SPITI VALLEY OF HIMACHAL PRADESHen_US
dc.typeThesisen_US
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