COMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINES

Simple item page
dc.contributor.advisorBinu K. Mani
dc.contributor.authorRESHMA S.
dc.date.accessioned2020-09-23T05:56:32Z
dc.date.available2020-09-23T05:56:32Z
dc.date.issued2019
dc.description.abstractThe present research was undertaken to compare the efficacy of LAMP and real time PCR with immunochromatography for diagnosis of brucellosis in bovines. One hundred and one samples were collected from University Livestock farms as well as from private cattle farms in and around Thrissur. The samples collected were aborted foetal internal organs and stomach contents, uterine discharges from aborted dams within two weeks after abortion and vaginal discharges from repeat breeder animals. All the samples were subjected to immunochromatography, LAMP and real time PCR. Among 101 samples tested, 8 (7.92 per cent) were found to be positive by immunochromatography; 19 (18.80 per cent) were found to be positive by real time PCR and 21 (20.79 per cent) were found to be positive by LAMP. However, LAMP showed a higher sensitivity (100 per cent) and specificity (86 per cent) in comparison to real time PCR (sensitivity 50 per cent and specificity 83.9 per cent) by taking immunochromatography as the standard test. More number of positive cases was reported from private farms compared to government farms when analysed by LAMP and real time PCR. Uterine discharges collected from aborted dams and the specimens from aborted foetus showed higher proportion of positive cases compared to vaginal discharges from repeat breeder animals.en_US
dc.identifier.urihttps://krishikosh.egranth.ac.in/handle/1/5810151816
dc.keywordsCOMPARATIVE EFFICACY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION IMMUNOCHROMATOGRAPHY DETECTION Brucella BOVINESen_US
dc.language.isoEnglishen_US
dc.pages88en_US
dc.publisherCollege of Veterinary and animal Science,Mannuthyen_US
dc.research.problemCOMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINESen_US
dc.subVeterinary Microbiologyen_US
dc.themeCOMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINESen_US
dc.these.typeM.V.Sc.en_US
dc.titleCOMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINESen_US
dc.typeThesisen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
RESHMA S..pdf
Size:
8.54 MB
Format:
Adobe Portable Document Format
Description:
COMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINES
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Thesis