COMPARATIVE EFFICACY OF LOOP-MEDIATED ISOTHERMAL AMPLIFICATION, REAL TIME POLYMERASE CHAIN REACTION AND IMMUNOCHROMATOGRAPHY FOR THE DETECTION OF Brucella IN BOVINES
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Date
2019
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College of Veterinary and animal Science,Mannuthy
Abstract
The present research was undertaken to compare the efficacy of LAMP and
real time PCR with immunochromatography for diagnosis of brucellosis in bovines.
One hundred and one samples were collected from University Livestock farms as well
as from private cattle farms in and around Thrissur. The samples collected were
aborted foetal internal organs and stomach contents, uterine discharges from aborted
dams within two weeks after abortion and vaginal discharges from repeat breeder
animals. All the samples were subjected to immunochromatography, LAMP and real
time PCR. Among 101 samples tested, 8 (7.92 per cent) were found to be positive by
immunochromatography; 19 (18.80 per cent) were found to be positive by real time
PCR and 21 (20.79 per cent) were found to be positive by LAMP. However, LAMP
showed a higher sensitivity (100 per cent) and specificity (86 per cent) in comparison
to real time PCR (sensitivity 50 per cent and specificity 83.9 per cent) by taking
immunochromatography as the standard test. More number of positive cases was
reported from private farms compared to government farms when analysed by LAMP
and real time PCR. Uterine discharges collected from aborted dams and the
specimens from aborted foetus showed higher proportion of positive cases compared
to vaginal discharges from repeat breeder animals.